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dc.contributor.authorWang, Ke
dc.contributor.authorLiu, Tzu-Ming
dc.contributor.authorWu, Juwell
dc.contributor.authorHorton, Nicholas G.
dc.contributor.authorLin, Charles P.
dc.contributor.authorXu, Chris
dc.date.accessioned2013-03-19T19:59:11Z
dc.date.issued2012
dc.identifier.citationWang, Ke, Tzu-Ming Liu, Juwell Wu, Nicholas G. Horton, Charles P. Lin, and Chris Xu. 2012. Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy. Biomedical Optics Express 3(9): 1972-1977.en_US
dc.identifier.issn2156-7085en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10448706
dc.description.abstractWe demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-nm pump laser and the 1728-nm and 1900-nm solitons generated through soliton self-frequency shift (SSFS) in a large-mode-area (LMA) fiber. These energetic pulses are well matched to the two-photon excitation peaks of red, cyan and yellow fluorescent proteins (TagRFPs, TagCFPs, and TagYFPs) for efficient excitation. We demonstrate simultaneous 2PM of human melanoma cells expressing a “rainbow” combination of these three fluorescent proteins.en_US
dc.language.isoen_USen_US
dc.publisherOptical Society of Americaen_US
dc.relation.isversionofdoi:10.1364/BOE.3.001972en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447541/pdf/en_US
dash.licenseLAA
dc.titleThree-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBiomedical Optics Expressen_US
dash.depositing.authorLin, Charles P.
dc.date.available2013-03-19T19:59:11Z
dc.identifier.doi10.1364/BOE.3.001972*
dash.contributor.affiliatedLiu, Tzu-Ming
dash.contributor.affiliatedWu, Juwell
dash.contributor.affiliatedLin, Charles


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