Multicolored Stain-Free Histopathology with Coherent Raman Imaging

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Multicolored Stain-Free Histopathology with Coherent Raman Imaging

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Title: Multicolored Stain-Free Histopathology with Coherent Raman Imaging
Author: Xie, Xiaoliang Sunney; Freudiger, Christian Wilhelm; Orringer, Daniel A.; Saar, Brian G.; Ji, Minbiao; Zeng, Qing; Ottoboni, Linda; Ying, Wei; Waeber, Christian; Sims, John R.; De Jager, Philip Lawrence; Sagher, Oren; Philbert, Martin A.; Xu, Xiaoyin; Kesari, Santosh; Young, Geoffrey; Pfannl, Rolf

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Citation: Freudiger, Christian Wilhelm, Rolf Pfannl, Daniel A. Orringer, Brian G. Saar, Minbiao Ji, Qing Zeng, Linda Ottoboni, et al. 2012. Multicolored stain-free histopathology with coherent Raman imaging. Laboratory Investigation 92(10): 1492-1502.
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Abstract: Conventional histopathology with hematoxylin & eosin (H&E) has been the gold standard for histopathological diagnosis of a wide range of diseases. However, it is not performed in vivo and requires thin tissue sections obtained after tissue biopsy, which carries risk, particularly in the central nervous system. Here we describe the development of an alternative, multicolored way to visualize tissue in real-time through the use of coherent Raman imaging (CRI), without the use of dyes. CRI relies on intrinsic chemical contrast based on vibrational properties of molecules and intrinsic optical sectioning by nonlinear excitation. We demonstrate that multicolor images originating from \(CH_2\) and \(CH_3\) vibrations of lipids and protein, as well as two-photon absorption of hemoglobin, can be obtained with subcellular resolution from fresh tissue. These stain-free histopathological images show resolutions similar to those obtained by conventional techniques, but do not require tissue fixation, sectioning or staining of the tissue analyzed.
Published Version: doi:10.1038/labinvest.2012.109
Other Sources: http://www.ncbi.nlm.nih.gov/pubmed/22906986
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10459028
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