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dc.contributor.authorXie, Xiaoliang Sunney
dc.contributor.authorFreudiger, Christian Wilhelm
dc.contributor.authorOrringer, Daniel A.
dc.contributor.authorSaar, Brian G.
dc.contributor.authorJi, Minbiao
dc.contributor.authorZeng, Qing
dc.contributor.authorOttoboni, Linda
dc.contributor.authorYing, Wei
dc.contributor.authorWaeber, Christian
dc.contributor.authorSims, John R.
dc.contributor.authorDe Jager, Philip Lawrence
dc.contributor.authorSagher, Oren
dc.contributor.authorPhilbert, Martin A.
dc.contributor.authorXu, Xiaoyin
dc.contributor.authorKesari, Santosh
dc.contributor.authorYoung, Geoffrey
dc.contributor.authorPfannl, Rolf
dc.date.accessioned2013-03-22T18:16:49Z
dc.date.issued2012
dc.identifier.citationFreudiger, Christian Wilhelm, Rolf Pfannl, Daniel A. Orringer, Brian G. Saar, Minbiao Ji, Qing Zeng, Linda Ottoboni, et al. 2012. Multicolored stain-free histopathology with coherent Raman imaging. Laboratory Investigation 92(10): 1492-1502.en_US
dc.identifier.issn0023-6837en_US
dc.identifier.issn1530-0307en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10459028
dc.description.abstractConventional histopathology with hematoxylin & eosin (H&E) has been the gold standard for histopathological diagnosis of a wide range of diseases. However, it is not performed in vivo and requires thin tissue sections obtained after tissue biopsy, which carries risk, particularly in the central nervous system. Here we describe the development of an alternative, multicolored way to visualize tissue in real-time through the use of coherent Raman imaging (CRI), without the use of dyes. CRI relies on intrinsic chemical contrast based on vibrational properties of molecules and intrinsic optical sectioning by nonlinear excitation. We demonstrate that multicolor images originating from \(CH_2\) and \(CH_3\) vibrations of lipids and protein, as well as two-photon absorption of hemoglobin, can be obtained with subcellular resolution from fresh tissue. These stain-free histopathological images show resolutions similar to those obtained by conventional techniques, but do not require tissue fixation, sectioning or staining of the tissue analyzed.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/labinvest.2012.109en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/22906986en_US
dash.licenseOAP
dc.subjectcoherent anti-Stokes Raman scatteringen_US
dc.subjectCARSen_US
dc.subjecthistologyen_US
dc.subjectin vivo microscopyen_US
dc.subjectstimulated Raman scatteringen_US
dc.subjectSRSen_US
dc.titleMulticolored Stain-Free Histopathology with Coherent Raman Imagingen_US
dc.typeJournal Articleen_US
dc.description.versionAuthor's Originalen_US
dc.relation.journalLaboratory Investigationen_US
dash.depositing.authorXie, Xiaoliang Sunney
dc.date.available2013-03-22T18:16:49Z
dc.identifier.doi10.1038/labinvest.2012.109*
dash.authorsorderedfalse
dash.contributor.affiliatedOttoboni, Linda
dash.contributor.affiliatedFreudiger, Christian Wilhelm
dash.contributor.affiliatedJi, Minbiao
dash.contributor.affiliatedXu, Xiaoyin
dash.contributor.affiliatedXie, Xiaoliang
dash.contributor.affiliatedYoung, Geoffrey
dash.contributor.affiliatedDe Jager, Philip


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