Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy

DSpace/Manakin Repository

Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy

Citable link to this page

 

 
Title: Coherent Nonlinear Optical Imaging: Beyond Fluorescence Microscopy
Author: Xie, Xiaoliang Sunney; Min, Wei; Freudiger, Christian Wilhelm; Lu, Sijia

Note: Order does not necessarily reflect citation order of authors.

Citation: Min, Wei, Christian Wilhelm Freudiger, Sijia Lu, and Xiaoliang Sunney Xie. 2011. Coherent nonlinear optical imaging: Beyond fluorescence microscopy. Annual Review of Physical Chemistry 62:507-530.
Full Text & Related Files:
Abstract: The quest for ultrahigh detection sensitivity with spectroscopic contrasts other than fluorescence has led to various novel approaches to optical microscopy of biological systems. Coherent nonlinear optical imaging, especially the recently developed nonlinear dissipation microscopy (including stimulated Raman scattering and two-photon absorption) and pump-probe microscopy (including excited-state absorption, stimulated emission, and ground-state depletion), provides new image contrasts for nonfluorescent species. Thanks to the high-frequency modulation transfer scheme, these imaging techniques exhibit superb detection sensitivity. By directly interrogating vibrational and/or electronic energy levels of molecules, they offer high molecular specificity. Here we review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques.
Published Version: doi:10.1146/annurev.physchem.012809.103512
Other Sources: http://www.ncbi.nlm.nih.gov/pubmed/21453061
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10461276
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters