A Genome-Wide Screen Identifies Genes That Affect Somatic Homolog Pairing in Drosophila

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A Genome-Wide Screen Identifies Genes That Affect Somatic Homolog Pairing in Drosophila

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Title: A Genome-Wide Screen Identifies Genes That Affect Somatic Homolog Pairing in Drosophila
Author: Bateman, Jack R.; Larschan, Erica; D’Souza, Ryan; Johnson, Justine E.; Mellone, Barbara G.; Marshall, Lauren S.; Dempsey, Kyle Edward; Kuroda, Mitzi I.

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Citation: Bateman, Jack R., Erica Larschan, Ryan D’Souza, Lauren S. Marshall, Kyle E. Dempsey, Justine E. Johnson, Barbara G. Mellone, and Mitzi I. Kuroda. 2012. A genome-wide screen identifies genes that affect somatic homolog pairing in drosophila. G3: Genes, Genomes, Genetics 2(7): 731-740.
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Abstract: In Drosophila and other Dipterans, homologous chromosomes are in close contact in virtually all nuclei, a phenomenon known as somatic homolog pairing. Although homolog pairing has been recognized for over a century, relatively little is known about its regulation. We performed a genome-wide RNAi-based screen that monitored the X-specific localization of the male-specific lethal (MSL) complex, and we identified 59 candidate genes whose knockdown via RNAi causes a change in the pattern of MSL staining that is consistent with a disruption of X-chromosomal homolog pairing. Using DNA fluorescent in situ hybridization (FISH), we confirmed that knockdown of 17 of these genes has a dramatic effect on pairing of the 359 bp repeat at the base of the X. Furthermore, dsRNAs targeting Pr-set7, which encodes an H4K20 methyltransferase, cause a modest disruption in somatic homolog pairing. Consistent with our results in cultured cells, a classical mutation in one of the strongest candidate genes, pebble (pbl), causes a decrease in somatic homolog pairing in developing embryos. Interestingly, many of the genes identified by our screen have known roles in diverse cell-cycle events, suggesting an important link between somatic homolog pairing and the choreography of chromosomes during the cell cycle.
Published Version: doi:10.1534/g3.112.002840
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3385979/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10465036
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