A Genome-Wide Screen Identifies Genes That Affect Somatic Homolog Pairing in Drosophila
Bateman, Jack R.
Marshall, Lauren S.
Johnson, Justine E.
Mellone, Barbara G.
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CitationBateman, Jack R., Erica Larschan, Ryan D’Souza, Lauren S. Marshall, Kyle E. Dempsey, Justine E. Johnson, Barbara G. Mellone, and Mitzi I. Kuroda. 2012. A genome-wide screen identifies genes that affect somatic homolog pairing in drosophila. G3: Genes, Genomes, Genetics 2(7): 731-740.
AbstractIn Drosophila and other Dipterans, homologous chromosomes are in close contact in virtually all nuclei, a phenomenon known as somatic homolog pairing. Although homolog pairing has been recognized for over a century, relatively little is known about its regulation. We performed a genome-wide RNAi-based screen that monitored the X-specific localization of the male-specific lethal (MSL) complex, and we identified 59 candidate genes whose knockdown via RNAi causes a change in the pattern of MSL staining that is consistent with a disruption of X-chromosomal homolog pairing. Using DNA fluorescent in situ hybridization (FISH), we confirmed that knockdown of 17 of these genes has a dramatic effect on pairing of the 359 bp repeat at the base of the X. Furthermore, dsRNAs targeting Pr-set7, which encodes an H4K20 methyltransferase, cause a modest disruption in somatic homolog pairing. Consistent with our results in cultured cells, a classical mutation in one of the strongest candidate genes, pebble (pbl), causes a decrease in somatic homolog pairing in developing embryos. Interestingly, many of the genes identified by our screen have known roles in diverse cell-cycle events, suggesting an important link between somatic homolog pairing and the choreography of chromosomes during the cell cycle.
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