Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons

DSpace/Manakin Repository

Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons

Citable link to this page

 

 
Title: Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons
Author: Saunders, Arpiar Bruce; Johnson, Caroline; Sabatini, Bernardo Luis

Note: Order does not necessarily reflect citation order of authors.

Citation: Saunders, Arpiar, Caroline A. Johnson, and Bernardo L. Sabatini. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons. Frontiers in Neural Circuits 6:47.
Full Text & Related Files:
Abstract: Understanding the organization of the nervous system requires methods for dissecting the contributions of each component cell type to circuit function. One widely used approach combines genetic targeting of Cre recombinase to specific cell populations with infection of recombinant adeno-associated viruses (rAAVs) whose transgene expression is activated by Cre (“Cre-On”). Distinguishing how the Cre-expressing neurons differ functionally from neighboring Cre-negative neurons requires rAAVs that are inactivated by Cre (“Cre-Off”) and can be used in tandem with Cre-On viruses. Here we introduce two rAAV vectors that are inactivated by Cre and carry different fluorophore and optogenetic constructs. We demonstrate single and dual rAAV systems to achieve Cre-On and Cre-Off expression in spatially-intermingled cell populations of the striatum. Using these systems, we uncovered cryptic genomic interactions that occur between multiple Cre-sensitive rAAVs or between Cre-sensitive rAAVs and somatic Cre-conditional alleles and devised methods to avoid these interactions. Our data highlight both important experimental caveats associated with Cre-dependent rAAV use as well as opportunities for the development of improved rAAVs for gene delivery.
Published Version: doi:10.3389/fncir.2012.00047
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406316/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10465988
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters