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dc.contributor.authorZhu, Lei
dc.contributor.authorGuo, Ning
dc.contributor.authorLi, Quanzheng
dc.contributor.authorMa, Ying
dc.contributor.authorJacboson, Orit
dc.contributor.authorLee, Seulki
dc.contributor.authorChoi, Hak Soo
dc.contributor.authorMansfield, James R.
dc.contributor.authorNiu, Gang
dc.contributor.authorChen, Xiaoyuan
dc.date.accessioned2013-03-29T15:38:09Z
dc.date.issued2012
dc.identifier.citationZhu, Lei, Ning Guo, Quanzheng Li, Ying Ma, Orit Jacboson, Seulki Lee, Hak Soo Choi, James R. Mansfield, Gang Niu, and Xiaoyuan Chen. 2012. Dynamic PET and optical imaging and compartment modeling using a dual-labeled cyclic RGD peptide probe. Theranostics 2(8): 746-756.en_US
dc.identifier.issn1838-7640en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10473957
dc.description.abstractPurpose: The aim of this study is to determine if dynamic optical imaging could provide comparable kinetic parameters to that of dynamic PET imaging by a near-infrared dye/64Cu dual-labeled cyclic RGD peptide. Methods: The integrin αvβ3 binding RGD peptide was conjugated with a macrocyclic chelator 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) for copper labeling and PET imaging and a near-infrared dye ZW-1 for optical imaging. The in vitro biological activity of RGD-C(DOTA)-ZW-1 was characterized by cell staining and receptor binding assay. Sixty-min dynamic PET and optical imaging were acquired on a MDA-MB-435 tumor model. Singular value decomposition (SVD) method was applied to compute the dynamic optical signal from the two-dimensional optical projection images. Compartment models were used to quantitatively analyze and compare the dynamic optical and PET data. Results: The dual-labeled probe 64Cu-RGD-C(DOTA)-ZW-1 showed integrin specific binding in vitro and in vivo. The binding potential (Bp) derived from dynamic optical imaging (1.762 ± 0.020) is comparable to that from dynamic PET (1.752 ± 0.026). Conclusion: The signal un-mixing process using SVD improved the accuracy of kinetic modeling of 2D dynamic optical data. Our results demonstrate that 2D dynamic optical imaging with SVD analysis could achieve comparable quantitative results as dynamic PET imaging in preclinical xenograft models.en_US
dc.language.isoen_USen_US
dc.publisherIvyspring International Publisheren_US
dc.relation.isversionofdoi:10.7150/thno.4762en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425122/pdf/en_US
dash.licenseLAA
dc.titleDynamic PET and Optical Imaging and Compartment Modeling using a Dual-labeled Cyclic RGD Peptide Probeen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalTheranosticsen_US
dash.depositing.authorLi, Quanzheng
dc.date.available2013-03-29T15:38:09Z
dc.identifier.doi10.7150/thno.4762*
dash.contributor.affiliatedLi, Quanzheng
dash.contributor.affiliatedChoi, Hak Soo


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