dc.contributor.author | Egydio de Carvalho, Carlos | |
dc.contributor.author | Van Bostelen, Ivo | |
dc.contributor.author | Gu, Yanjie | |
dc.contributor.author | Chu, Diana S. | |
dc.contributor.author | Cheeseman, Iain M. | |
dc.contributor.author | Tzur, Yonatan B | |
dc.contributor.author | Nadarajan, Saravanapriah | |
dc.contributor.author | Colaiacovo, Monica P. | |
dc.date.accessioned | 2013-03-29T18:16:13Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Tzur, Yonatan B., Carlos Egydio de Carvalho, Saravanapriah Nadarajan, Ivo Van Bostelen, Yanjie Gu, Diana S. Chu, Iain M. Cheeseman, and Monica P. Colaiácovo. 2012. LAB-1 targets PP1 and restricts Aurora B Kinase upon entrance into meiosis to promote sister chromatid cohesion. PLoS Biology 10(8): e1001378. | en_US |
dc.identifier.issn | 1544-9173 | en_US |
dc.identifier.issn | 1545-7885 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10474257 | |
dc.description.abstract | Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.isversionof | doi:10.1371/journal.pbio.1001378 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424243/pdf/ | en_US |
dash.license | LAA | |
dc.subject | Biology | en_US |
dc.title | LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | PLoS Biology | en_US |
dash.depositing.author | Tzur, Yonatan B | |
dc.date.available | 2013-03-29T18:16:13Z | |
dc.identifier.doi | 10.1371/journal.pbio.1001378 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Tzur, Yonatan B | |
dash.contributor.affiliated | Nadarajan, Saravanapriah | |
dash.contributor.affiliated | Colaiacovo, Monica | |