Show simple item record

dc.contributor.authorEgydio de Carvalho, Carlos
dc.contributor.authorVan Bostelen, Ivo
dc.contributor.authorGu, Yanjie
dc.contributor.authorChu, Diana S.
dc.contributor.authorCheeseman, Iain M.
dc.contributor.authorTzur, Yonatan B
dc.contributor.authorNadarajan, Saravanapriah
dc.contributor.authorColaiacovo, Monica P.
dc.date.accessioned2013-03-29T18:16:13Z
dc.date.issued2012
dc.identifier.citationTzur, Yonatan B., Carlos Egydio de Carvalho, Saravanapriah Nadarajan, Ivo Van Bostelen, Yanjie Gu, Diana S. Chu, Iain M. Cheeseman, and Monica P. Colaiácovo. 2012. LAB-1 targets PP1 and restricts Aurora B Kinase upon entrance into meiosis to promote sister chromatid cohesion. PLoS Biology 10(8): e1001378.en_US
dc.identifier.issn1544-9173en_US
dc.identifier.issn1545-7885en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10474257
dc.description.abstractSuccessful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pbio.1001378en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424243/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.titleLAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesionen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Biologyen_US
dash.depositing.authorTzur, Yonatan B
dc.date.available2013-03-29T18:16:13Z
dc.identifier.doi10.1371/journal.pbio.1001378*
dash.authorsorderedfalse
dash.contributor.affiliatedTzur, Yonatan B
dash.contributor.affiliatedNadarajan, Saravanapriah
dash.contributor.affiliatedColaiacovo, Monica


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record