3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleus

DSpace/Manakin Repository

3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleus

Citable link to this page

 

 
Title: 3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleus
Author: Poczatek, J. Collin; Lechene, Claude P.; Lee, Gloria Y.; Toner, Mehmet; Biggers, John Dennis

Note: Order does not necessarily reflect citation order of authors.

Citation: Lechene, Claude P., Gloria Y. Lee, J. Collin Poczatek, Mehmet Toner, and John D. Biggers. 2012. 3D multi-isotope imaging mass spectrometry reveals penetration of \(^{18}O\)-trehalose in mouse sperm nucleus. PLoS ONE 7(8): e42267.
Full Text & Related Files:
Abstract: The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.
Published Version: doi:10.1371/journal.pone.0042267
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428319/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10482571
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters