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dc.contributor.authorLechene, Claude P.
dc.contributor.authorLee, Gloria Y.
dc.contributor.authorPoczatek, J. Collin
dc.contributor.authorToner, Mehmet
dc.contributor.authorBiggers, John Dennis
dc.date.accessioned2013-03-29T19:57:24Z
dc.date.issued2012
dc.identifier.citationLechene, Claude P., Gloria Y. Lee, J. Collin Poczatek, Mehmet Toner, and John D. Biggers. 2012. 3D multi-isotope imaging mass spectrometry reveals penetration of \(^{18}O\)-trehalose in mouse sperm nucleus. PLoS ONE 7(8): e42267.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10482571
dc.description.abstractThe prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0042267en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428319/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectAnatomy and Physiologyen_US
dc.subjectCell Physiologyen_US
dc.subjectReproductive Systemen_US
dc.subjectBiotechnologyen_US
dc.subjectGenetic Engineeringen_US
dc.subjectTransgenicsen_US
dc.subjectCryobiologyen_US
dc.subjectDevelopmental Biologyen_US
dc.subjectMolecular Developmenten_US
dc.subjectHistologyen_US
dc.subjectModel Organismsen_US
dc.subjectAnimal Modelsen_US
dc.subjectMouseen_US
dc.title3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleusen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorToner, Mehmet
dc.date.available2013-03-29T19:57:24Z
dc.identifier.doi10.1371/journal.pone.0042267*
dash.contributor.affiliatedBiggers, John
dash.contributor.affiliatedLee, Gloria
dash.contributor.affiliatedLechene, Claude
dash.contributor.affiliatedToner, Mehmet


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