R5-SHIV Induces Multiple Defects in T Cell Function during Early Infection of Rhesus Macaques Including Accumulation of T Reg Cells in Lymph Nodes
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Righi, Elda
Leblanc, Pierre R.
Kodish, Brett
Mylvaganam, Hari N.
Stevceva, Liljana
Hu, Shiu-Lok
Chenine, Agnes-L.
Hovav, Avi-Hai
Unutmaz, Derya
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1371/journal.pone.0018465Metadata
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Santosuosso, Michael, Elda Righi, E. David Hill, Pierre R. Leblanc, Brett Kodish, Hari N. Mylvaganam, Nagadenahalli B. Siddappa et al. 2011. R5-SHIV induces multiple defects in T Cell function during early infection of Rhesus macaques including accumulation of T Reg Cells in lymph nodes. PLoS ONE 6(4): e18465Abstract
Background: HIV-1 is a pathogen that T cell responses fail to control. HIV-1gp120 is the surface viral envelope glycoprotein that interacts with CD4 T cells and mediates entry. HIV-1gp120 has been implicated in immune dysregulatory functions that may limit anti-HIV antigen-specific T cell responses. We hypothesized that in the context of early SHIV infection, immune dysregulation of antigen-specific T-effector cell and regulatory functions would be detectable and that these would be associated or correlated with measurable concentrations of HIV-1gp120 in lymphoid tissues. Methods: Rhesus macaques were intravaginally inoculated with a Clade C CCR5-tropic simian-human immunodeficiency virus, SHIV-1157ipd3N4. HIV-1gp120 levels, antigen-specificity, levels of apoptosis/anergy and frequency and function of Tregs were examined in lymph node and blood derived T cells at 5 and 12 weeks post inoculation. Results/Conclusions: We observed reduced responses to Gag in CD4 and gp120 in CD8 lymph node-derived T cells compared to the peripheral blood at 5 weeks post-inoculation. Reduced antigen-specific responses were associated with higher levels of PD-1 on lymph node-derived CD4 T cells as compared to peripheral blood and uninfected lymph node-derived CD4 T cells. Lymph nodes contained increased numbers of Tregs as compared to peripheral blood, which positively correlated with gp120 levels; T regulatory cell depletion restored CD8 T cell responses to Gag but not to gp120. HIV gp120 was also able to induce T regulatory cell chemotaxis in a dose-dependent, CCR5-mediated manner. These studies contribute to our broader understanding of the ways in which HIV-1 dysregulates T cell function and localization during early infection.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071731/pdf/Terms of Use
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