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dc.contributor.authorAagaard, Kjersti
dc.contributor.authorRiehle, Kevin
dc.contributor.authorMa, Jun
dc.contributor.authorSegata, Nicola
dc.contributor.authorMistretta, Toni-Ann
dc.contributor.authorCoarfa, Cristian
dc.contributor.authorRaza, Sabeen
dc.contributor.authorRosenbaum, Sean
dc.contributor.authorVan den Veyver, Ignatia
dc.contributor.authorMilosavljevic, Aleksandar
dc.contributor.authorGevers, Dirk
dc.contributor.authorHuttenhower, Curtis
dc.contributor.authorPetrosino, Joseph
dc.contributor.authorVersalovic, James
dc.date.accessioned2013-04-03T19:15:51Z
dc.date.issued2012
dc.identifier.citationAagaard, Kjersti, Kevin Riehle, Jun Ma, Nicola Segata, Toni-Ann Mistretta, Cristian Coarfa, Sabeen Raza, Sean Rosenbaum, Ignatia Van den Veyver, Aleksandar Milosavljevic, Dirk Gevers, Curtis Huttenhower, Joseph Petrosino, and James Versalovic. 2012. A metagenomic approach to characterization of the vaginal microbiome signature in pregnancy. PLoS ONE 7(6): e36466.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10497277
dc.description.abstractWhile current major national research efforts (i.e., the NIH Human Microbiome Project) will enable comprehensive metagenomic characterization of the adult human microbiota, how and when these diverse microbial communities take up residence in the host and during reproductive life are unexplored at a population level. Because microbial abundance and diversity might differ in pregnancy, we sought to generate comparative metagenomic signatures across gestational age strata. DNA was isolated from the vagina (introitus, posterior fornix, midvagina) and the V5V3 region of bacterial 16S rRNA genes were sequenced (454FLX Titanium platform). Sixty-eight samples from 24 healthy gravidae (18 to 40 confirmed weeks) were compared with 301 non-pregnant controls (60 subjects). Generated sequence data were quality filtered, taxonomically binned, normalized, and organized by phylogeny and into operational taxonomic units (OTU); principal coordinates analysis (PCoA) of the resultant beta diversity measures were used for visualization and analysis in association with sample clinical metadata. Altogether, 1.4 gigabytes of data containing >2.5 million reads (averaging 6,837 sequences/sample of 493 nt in length) were generated for computational analyses. Although gravidae were not excluded by virtue of a posterior fornix pH >4.5 at the time of screening, unique vaginal microbiome signature encompassing several specific OTUs and higher-level clades was nevertheless observed and confirmed using a combination of phylogenetic, non-phylogenetic, supervised, and unsupervised approaches. Both overall diversity and richness were reduced in pregnancy, with dominance of Lactobacillus species (L. iners crispatus, jensenii and johnsonii, and the orders Lactobacillales (and Lactobacillaceae family), Clostridiales, Bacteroidales, and Actinomycetales. This intergroup comparison using rigorous standardized sampling protocols and analytical methodologies provides robust initial evidence that the vaginal microbial 16S rRNA gene catalogue uniquely differs in pregnancy, with variance of taxa across vaginal subsite and gestational age.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0036466en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3374618/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectComputational Biologyen_US
dc.subjectGenomicsen_US
dc.subjectMetagenomicsen_US
dc.subjectMicrobiologyen_US
dc.subjectApplied Microbiologyen_US
dc.subjectMedical Microbiologyen_US
dc.subjectMicrobial Pathogensen_US
dc.subjectMedicineen_US
dc.subjectInfectious Diseasesen_US
dc.subjectGynecologic Infectionsen_US
dc.subjectInfectious Disease Modelingen_US
dc.subjectObstetrics and Gynecologyen_US
dc.subjectPregnancyen_US
dc.subjectPregnancy Complicationsen_US
dc.subjectPreterm Laboren_US
dc.subjectFemale Genital Diseasesen_US
dc.subjectGenitourinary Infectionsen_US
dc.titleA Metagenomic Approach to Characterization of the Vaginal Microbiome Signature in Pregnancyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorHuttenhower, Curtis
dc.date.available2013-04-03T19:15:51Z
dc.identifier.doi10.1371/journal.pone.0036466*
dash.contributor.affiliatedHuttenhower, Curtis
dc.identifier.orcid0000-0002-1110-0096


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