Show simple item record

dc.contributor.authorCaboux, Elodie
dc.contributor.authorLallemand, Christophe
dc.contributor.authorFerro, Gilles
dc.contributor.authorHémon, Bertrand
dc.contributor.authorMendy, Maimuna
dc.contributor.authorBiessy, Carine
dc.contributor.authorSims, Matt
dc.contributor.authorWareham, Nick
dc.contributor.authorBritten, Abigail
dc.contributor.authorBoland, Anne
dc.contributor.authorHutchinson, Amy
dc.contributor.authorSiddiq, Afshan
dc.contributor.authorVineis, Paolo
dc.contributor.authorRiboli, Elio
dc.contributor.authorRomieu, Isabelle
dc.contributor.authorRinaldi, Sabina
dc.contributor.authorGunter, Marc J.
dc.contributor.authorPeeters, Petra H. M.
dc.contributor.authorvan der Schouw, Yvonne T.
dc.contributor.authorTravis, Ruth
dc.contributor.authorBueno-de-Mesquita, H. Bas
dc.contributor.authorCanzian, Federico
dc.contributor.authorSánchez, Maria-José
dc.contributor.authorSkeie, Guri
dc.contributor.authorOlsen, Karina Standahl
dc.contributor.authorLund, Eiliv
dc.contributor.authorBilbao, Roberto
dc.contributor.authorSala, Núria
dc.contributor.authorBarricarte, Aurelio
dc.contributor.authorPalli, Domenico
dc.contributor.authorNavarro, Carmen
dc.contributor.authorPanico, Salvatore
dc.contributor.authorRedondo, Maria Luisa
dc.contributor.authorPolidoro, Silvia
dc.contributor.authorDossus, Laure
dc.contributor.authorBoutron-Ruault, Marie Christine
dc.contributor.authorClavel-Chapelon, Françoise
dc.contributor.authorTrichopoulou, Antonia
dc.contributor.authorTrichopoulos, Dimitrios
dc.contributor.authorLagiou, Pagona
dc.contributor.authorBoeing, Heiner
dc.contributor.authorFisher, Eva
dc.contributor.authorTumino, Rosario
dc.contributor.authorAgnoli, Claudia
dc.contributor.authorHainaut, Pierre
dc.date.accessioned2013-04-03T20:46:42Z
dc.date.issued2012
dc.identifier.citationCaboux, Elodie, Christophe Lallemand, Gilles Ferro, Bertrand Hémon, Maimuna Mendy, Carine Biessy, Matt Sims, et al. 2012. Sources of pre-analytical variations in yield of DNA extracted from blood samples: analysis of 50,000 DNA samples in EPIC. PLoS ONE 7(7): e39821.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10497294
dc.description.abstractThe European Prospective Investigation into Cancer and nutrition (EPIC) is a long-term, multi-centric prospective study in Europe investigating the relationships between cancer and nutrition. This study has served as a basis for a number of Genome-Wide Association Studies (GWAS) and other types of genetic analyses. Over a period of 5 years, 52,256 EPIC DNA samples have been extracted using an automated DNA extraction platform. Here we have evaluated the pre-analytical factors affecting DNA yield, including anthropometric, epidemiological and technical factors such as center of subject recruitment, age, gender, body-mass index, disease case or control status, tobacco consumption, number of aliquots of buffy coat used for DNA extraction, extraction machine or procedure, DNA quantification method, degree of haemolysis and variations in the timing of sample processing. We show that the largest significant variations in DNA yield were observed with degree of haemolysis and with center of subject recruitment. Age, gender, body-mass index, cancer case or control status and tobacco consumption also significantly impacted DNA yield. Feedback from laboratories which have analyzed DNA with different SNP genotyping technologies demonstrate that the vast majority of samples (approximately 88%) performed adequately in different types of assays. To our knowledge this study is the largest to date to evaluate the sources of pre-analytical variations in DNA extracted from peripheral leucocytes. The results provide a strong evidence-based rationale for standardized recommendations on blood collection and processing protocols for large-scale genetic studies.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0039821en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3396633/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectNucleic Acidsen_US
dc.subjectDNAen_US
dc.subjectComputational Biologyen_US
dc.subjectGenomicsen_US
dc.subjectCryobiologyen_US
dc.subjectGeneticsen_US
dc.subjectGenome-Wide Association Studiesen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCellular Typesen_US
dc.subjectBlood Cellsen_US
dc.subjectPopulation Biologyen_US
dc.subjectEpidemiologyen_US
dc.subjectGenetic Epidemiologyen_US
dc.subjectMedicineen_US
dc.subjectClinical Research Designen_US
dc.titleSources of Pre-Analytical Variations in Yield of DNA Extracted from Blood Samples: Analysis of 50,000 DNA Samples in EPICen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorTrichopoulos, Dimitrios
dc.date.available2013-04-03T20:46:42Z
dc.identifier.doi10.1371/journal.pone.0039821*
dash.authorsorderedfalse
dash.contributor.affiliatedLagiou, Pagona
dash.contributor.affiliatedTrichopoulos, Dimitrios


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record