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dc.contributor.authorRullo, Jacob
dc.contributor.authorBecker, Henry
dc.contributor.authorHyduk, Sharon J.
dc.contributor.authorWong, Janice C.
dc.contributor.authorDigby, Genevieve
dc.contributor.authorArora, Pamma D.
dc.contributor.authorCano, Adrianet Puig
dc.contributor.authorHartwig, John Henry
dc.contributor.authorMcCulloch, Christopher A.
dc.contributor.authorCybulsky, Myron I.
dc.date.accessioned2013-04-05T18:56:25Z
dc.date.issued2012
dc.identifier.citationRullo, Jacob, Henry Becker, Sharon J. Hyduk, Janice C. Wong, Genevieve Digby, Pamma D. Arora, Adrianet Puig Cano, John Hartwig, Christopher A. McCulloch, and Myron I. Cybulsky. 2012. Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion. The Journal of Cell Biology 197(1): 115-129.en_US
dc.identifier.issn0021-9525en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10504656
dc.description.abstractLeukocytes arrested on inflamed endothelium via integrins are subjected to force imparted by flowing blood. How leukocytes respond to this force and resist detachment is poorly understood. Live-cell imaging with Lifeact-transfected U937 cells revealed that force triggers actin polymerization at upstream α4β1 integrin adhesion sites and the adjacent cortical cytoskeleton. Scanning electron microscopy revealed that this culminates in the formation of structures that anchor monocyte adhesion. Inhibition of actin polymerization resulted in cell deformation, displacement, and detachment. Transfection of dominant-negative constructs and inhibition of function or expression revealed key signaling steps required for upstream actin polymerization and adhesion stabilization. These included activation of Rap1, phosphoinositide 3-kinase γ isoform, and Rac but not Cdc42. Thus, rapid signaling and structural adaptations enable leukocytes to stabilize adhesion and resist detachment forces.en_US
dc.language.isoen_USen_US
dc.publisherThe Rockefeller University Pressen_US
dc.relation.isversionofdoi:10.1083/jcb.201107140en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317807/pdf/en_US
dash.licenseLAA
dc.titleActin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesionen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalThe Journal of Cell Biologyen_US
dash.depositing.authorHartwig, John Henry
dc.date.available2013-04-05T18:56:25Z
dc.identifier.doi10.1083/jcb.201107140*
dash.contributor.affiliatedWong, Janice C.
dash.contributor.affiliatedHartwig, John


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