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dc.contributor.authorHwang, Ji Sun
dc.contributor.authorKwon, Ho-Keun
dc.contributor.authorKim, Jung-Eun
dc.contributor.authorRho, Jeonghae
dc.contributor.authorIm, Sin-Hyeog
dc.date.accessioned2013-04-08T18:26:51Z
dc.date.issued2012
dc.identifier.citationHwang, Ji Sun, Ho-Keun Kwon, Jung-Eun Kim, Jeonghae Rho, and Sin-Hyeog Im. 2012. Immunomodulatory effect of water soluble extract separated from mycelium of Phellinus linteus on experimental atopic dermatitis. BMC Complementary and Alternative Medicine 12:159.en_US
dc.identifier.issn1472-6882en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10511083
dc.description.abstractBackground: Complementary and alternative medicine (CAM) is becoming a popular treatment for modulating diverse immune disorders. Phellinus linteus (P. linteus) as one of the CAMs has been used to modulate cancers, inflammation and allergic activities. However, little evidence has been shown about its underlying mechanism of action by which it exerts a beneficial role in dermatological disease in vivo. In this study, we examined the immunomodulatory effects of P. linteus on experimental atopic dermatitis (AD) and elucidated its action mechanism. Methods: The immunomodulatory effect of total extract of P. linteus on IgE production by human myeloma U266B1 cells was measured by ELISA. To further identify the effective components, P. linteus was fractionated into methanol soluble, water soluble and boiling water soluble extracts. Each extract was treated to U266B1 cells and primary B cells to compare their inhibitory effects on IgE secretion. To test the in vivo efficacy, experimental atopic dermatitis (AD) was established by alternative treatment of DNCB and house dust mite extract into BALB/c mice. Water soluble extract of P. linteus (WA) or ceramide as a positive control were topically applied to ears of atopic mouse every day for 2 weeks and progression of the disease was estimated by the following criteria: (a) ear thickness, clinical score, (b) serum total IgE, IgG and mite specific IgE level by ELSIA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total ear cells and CD4+ T cells by real time PCR and ELSIA. Results: Treatment of total extracts of P. linteus to U266B1 inhibited IgE secretion. Among the diverse extracts of P. linteus, water soluble extract of P. linteus (WA) significantly reduced the IgE production in primary B cells and B cell line U266B1. Moreover, treatment of WA reduced AD symptoms such as ear swelling, erythema, and dryness and decreased recruitment of lymphocyte into the inflamed site. Interestingly WA treatment significantly reduced IgE level without affecting IgG levels and also down-regulated the levels of pathogenic cytokines (IL-4, IL-13, IL-12 and IFN-γ) and chemokines (CCL17 and CCL22) involved in AD development. Conclusions: Our study indicates that protective effect of water soluble extract of P. linteus in atopic dermatitis is mediated by inhibiting IgE production and expression of AD associated pathogenic cytokines as well as chemokines, suggesting the beneficial effect of P. linteus to modulate allergic skin disease.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1472-6882-12-159en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479073/pdf/en_US
dash.licenseLAA
dc.titleImmunomodulatory effect of water soluble extract separated from mycelium of Phellinus linteus on experimental atopic dermatitisen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Complementary and Alternative Medicineen_US
dash.depositing.authorKwon, Ho-Keun
dc.date.available2013-04-08T18:26:51Z
dc.identifier.doi10.1186/1472-6882-12-159*
dash.contributor.affiliatedKwon, Ho-Keun


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