Identification and Characterization of Receptor-Specific Peptides for siRNA Delivery

DSpace/Manakin Repository

Identification and Characterization of Receptor-Specific Peptides for siRNA Delivery

Citable link to this page

 

 
Title: Identification and Characterization of Receptor-Specific Peptides for siRNA Delivery
Author: Ren, Yin; Hauert, Sabine; Lo, Justin Han-Je; Bhatia, Sangeeta

Note: Order does not necessarily reflect citation order of authors.

Citation: Ren, Yin, Sabine Hauert, Justin H. Lo, and Sangeeta N. Bhatia. 2012. Identification and characterization of receptor-specific peptides for siRNA delivery. ACS Nano 6(10): 8620-8631.
Full Text & Related Files:
Abstract: Tumor-targeted delivery of siRNA remains a major barrier in fully realizing the therapeutic potential of RNA interference. While cell-penetrating peptides (CPP) are promising siRNA carrier candidates, they are universal internalizers that lack cell-type specificity. Herein, we design and screen a library of tandem tumor-targeting and cell-penetrating peptides that condense siRNA into stable nanocomplexes for cell type-specific siRNA delivery. Through physiochemical and biological characterization, we identify a subset of the nanocomplex library of that are taken up by cells via endocytosis, trigger endosomal escape and unpacking of the carrier, and ultimately deliver siRNA to the cytosol in a receptor-specific fashion. To better understand the structure–activity relationships that govern receptor-specific siRNA delivery, we employ computational regression analysis and identify a set of key convergent structural properties, namely the valence of the targeting ligand and the charge of the peptide, that help transform ubiquitously internalizing cell-penetrating peptides into cell type-specific siRNA delivery systems.
Published Version: doi:10.1021/nn301975s
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478735/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10511099
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters