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dc.contributor.authorFine, Debrah A.
dc.contributor.authorRozenblatt-Rosen, Orit
dc.contributor.authorPadi, Megha
dc.contributor.authorKorkhin, Anna
dc.contributor.authorJames, Robert L.
dc.contributor.authorAdelmant, Guillaume
dc.contributor.authorYoon, Rosa
dc.contributor.authorGuo, Luxuan
dc.contributor.authorBerrios, Christian Jose
dc.contributor.authorZhang, Ying
dc.contributor.authorCalderwood, Michael A.
dc.contributor.authorVelmurgan, Soundarapandian
dc.contributor.authorCheng, Jingwei
dc.contributor.authorMarto, Jarrod
dc.contributor.authorHill, David E.
dc.contributor.authorCusick, Michael
dc.contributor.authorVidal, Marc
dc.contributor.authorFlorens, Laurence
dc.contributor.authorWashburn, Michael P.
dc.contributor.authorLitovchick, Larisa
dc.contributor.authorDeCaprio, James A.
dc.date.accessioned2013-04-08T18:57:31Z
dc.date.issued2012
dc.identifier.citationFine, Debrah A., Orit Rozenblatt-Rosen, Megha Padi, Anna Korkhin, Robert L. James, Guillaume Adelmant, Rosa Yoon, et al. 2012. Identification of FAM111A as an SV40 host range restriction and adenovirus helper factor. PLoS Pathogens 8(10): e1002949.en_US
dc.identifier.issn1553-7366en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10511331
dc.description.abstractThe small genome of polyomaviruses encodes a limited number of proteins that are highly dependent on interactions with host cell proteins for efficient viral replication. The SV40 large T antigen (LT) contains several discrete functional domains including the LXCXE or RB-binding motif, the DNA binding and helicase domains that contribute to the viral life cycle. In addition, the LT C-terminal region contains the host range and adenovirus helper functions required for lytic infection in certain restrictive cell types. To understand how LT affects the host cell to facilitate viral replication, we expressed full-length or functional domains of LT in cells, identified interacting host proteins and carried out expression profiling. LT perturbed the expression of p53 target genes and subsets of cell-cycle dependent genes regulated by the DREAM and the B-Myb-MuvB complexes. Affinity purification of LT followed by mass spectrometry revealed a specific interaction between the LT C-terminal region and FAM111A, a previously uncharacterized protein. Depletion of FAM111A recapitulated the effects of heterologous expression of the LT C-terminal region, including increased viral gene expression and lytic infection of SV40 host range mutants and adenovirus replication in restrictive cells. FAM111A functions as a host range restriction factor that is specifically targeted by SV40 LT.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.ppat.1002949en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475652/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectProteinsen_US
dc.subjectProtein Interactionsen_US
dc.subjectMicrobiologyen_US
dc.subjectVirologyen_US
dc.subjectVirulence Factors and Mechanismsen_US
dc.subjectHost-Pathogen Interactionen_US
dc.titleIdentification of FAM111A as an SV40 Host Range Restriction and Adenovirus Helper Factoren_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Pathogensen_US
dash.depositing.authorMarto, Jarrod
dc.date.available2013-04-08T18:57:31Z
dc.identifier.doi10.1371/journal.ppat.1002949*
dash.authorsorderedfalse
dash.contributor.affiliatedCheng, Jingwei
dash.contributor.affiliatedZhang, Ying
dash.contributor.affiliatedBerrios, Christian Jose
dash.contributor.affiliatedGuo, Luxuan
dash.contributor.affiliatedCusick, Michael
dash.contributor.affiliatedAdelmant, Guillaume
dash.contributor.affiliatedPadi, Megha
dash.contributor.affiliatedYoon, Rosa
dash.contributor.affiliatedCalderwood, Michael
dash.contributor.affiliatedDeCaprio, James
dash.contributor.affiliatedHill, David
dash.contributor.affiliatedVidal, Marc
dash.contributor.affiliatedMarto, Jarrod


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