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dc.contributor.authorSharma, Prasahant P.
dc.contributor.authorSchwager, Evelyn Elisabeth
dc.contributor.authorGiribet, Gonzalo
dc.contributor.authorJockusch, Elizabeth L.
dc.contributor.authorExtavour, Cassandra G.
dc.date.accessioned2013-04-08T19:57:23Z
dc.date.issued2013-04-08
dc.identifierQuick submit: 2013-03-22T20:27:19-04:00
dc.identifier.citationSharma, Prashant P., Evelyn Elisabeth Schwager, Gonzalo Giribet, Elizabeth L. Jockusch, and Cassandra G. Extavour. Forthcoming. Distal-less and dachshund pattern both plesiomorphic and apomorphic structures in chelicerates: RNA interference in the harvestman Phalangium opilio (Opiliones). Evolution and Development.en_US
dc.identifier.issn1520-541Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10512167
dc.description.abstractThe discovery of genetic mechanisms that can transform a morphological structure from a plesiomorphic (=primitive) state to an apomorphic (=derived) one is a cardinal objective of evolutionary developmental biology. However, this objective is often impeded for many lineages of interest by limitations in taxonomic sampling, genomic resources, or functional genetic methods. In order to investigate the evolution of appendage morphology within Chelicerata, the putative sister group of the remaining arthropods, we developed an RNA interference (RNAi) protocol for the harvestman Phalangium opilio. We silenced the leg gap genes Distal-less(Dll) and dachshund (dac) in the harvestman via zygotic injections of double-stranded RNA, and used in situ hybridization to confirm RNAi efficacy. Consistent with the conserved roles of these genes in patterning the proximo-distal axis of arthropod appendages, we observed that embryos injected with Dll dsRNA lacked distal parts of appendages and appendage-like structures, such as the labrum, the chelicerae, the pedipalps, and the walking legs, whereas embryos injected with dac dsRNA lacked the medial podomeres femur and patella in the pedipalps and walking legs. In addition, we detected a role for these genes in patterning structures that do not occur in well-established chelicerate models (spiders and mites). Dll RNAi additionally results in loss of the preoral chamber, which is formed from pedipalpal and leg coxapophyses, and the ocularium, a dorsal outgrowth bearing the eyes. In one case, we observed that an embryo injected with dac dsRNA lacked the proximal segment of the chelicera, a plesiomorphic podomere that expresses dac in wild type embryos. This may support the hypothesis that loss of the cheliceral dac domain underlies the transition to the two-segmented chelicera of derived arachnids.en_US
dc.description.sponsorshipOrganismic and Evolutionary Biologyen_US
dc.language.isoen_USen_US
dc.publisherWiley-Blackwellen_US
dash.licenseOAP
dc.subjectgene silencingen_US
dc.subjectRNAien_US
dc.subjectArachnidaen_US
dc.subjectChelicerataen_US
dc.subjectarthropod legen_US
dc.subjectleg gap genesen_US
dc.titleDistal-less and Dachshund Pattern both Plesiomorphic and Apomorphic Structures in Chelicerates: RNA Interference in the Harvestman Phalangium opilio (Opiliones)en_US
dc.typeJournal Articleen_US
dc.date.updated2013-03-23T00:27:25Z
dc.description.versionAccepted Manuscripten_US
dc.rights.holderSharma, P.P., Schwager, E.E., Giribet, G.G., Jockusch, E. and Extavour, C.
dc.relation.journalEvolution and Developmenten_US
dash.depositing.authorExtavour, Cassandra G.
dc.date.available2013-04-08T19:57:23Z
dc.identifier.doi10.1111/ede.12029
dash.contributor.affiliatedSchwager, Evelyn
dash.contributor.affiliatedExtavour, Cassandra
dash.contributor.affiliatedGiribet, Gonzalo


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