Single Molecule Detection of Direct, Homologous, DNA/DNA Pairing
Lee, C. H.
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CitationDanilowicz, Claudia, C. H. Lee, K. Kim, K. Hatch, Vincent William Coljee, Nancy Elizabeth Kleckner, and Mara Prentiss. 2009. Single molecule detection of direct, homologous, DNA/DNA pairing. Proceedings of the National Academy of Sciences of the United States of America 106(47): 19824-19829.
AbstractUsing a parallel single molecule magnetic tweezers assay we demonstrate homologous pairing of two double-stranded (ds) DNA molecules in the absence of proteins, divalent metal ions, crowding agents, or free DNA ends. Pairing is accurate and rapid under physiological conditions of temperature and monovalent salt, even atDNA molecule concentrations orders of magnitude below those found in vivo, and in the presence of a large excess of nonspecific competitor DNA. Crowding agents further increase the reaction rate. Pairing is readily detected between regions of homology of 5 kb or more. Detected pairs are stable against thermal forces and shear forces up to 10 pN. These results strongly suggest that direct recognition of homology between chemically intact B-DNA molecules should be possible in vivo. The robustness of the observed signal raises the possibility that pairing might even be the "default" option, limited to desired situations by specific features. Protein-independent homologous pairing of intact dsDNA has been predicted theoretically, but further studies are needed to determine whether existing theories fit sequence length, temperature, and salt dependencies described here.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10520825
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