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dc.contributor.authorRay, Arundhati
dc.contributor.authorAmato, Anthony A.
dc.contributor.authorBradshaw, Elizabeth M.
dc.contributor.authorFelice, Kevin J.
dc.contributor.authorDiCapua, Daniel B.
dc.contributor.authorGoldstein, Jonathan M.
dc.contributor.authorLundberg, Ingrid E.
dc.contributor.authorNowak, Richard J.
dc.contributor.authorPloegh, Hidde L.
dc.contributor.authorSpooner, Eric
dc.contributor.authorWu, Qian
dc.contributor.authorWillis, Simon N.
dc.contributor.authorO’Connor, Kevin C.
dc.date.accessioned2013-04-10T15:04:05Z
dc.date.issued2012
dc.identifier.citationRay, Arundhati, Anthony A. Amato, Elizabeth M. Bradshaw, Kevin J. Felice, Daniel B. DiCapua, Jonathan M. Goldstein, Ingrid E. Lundberg, et al. 2012. Autoantibodies produced at the site of tissue damage provide evidence of humoral autoimmunity in inclusion body myositis. PLoS ONE 7(10): e46709.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10522859
dc.description.abstractInclusion body myositis (IBM) belongs to a group of muscle diseases known as the inflammatory myopathies. The presence of antibody-secreting plasma cells in IBM muscle implicates the humoral immune response in this disease. However, whether the humoral immune response actively contributes to IBM pathology has not been established. We sought to investigate whether the humoral immune response in IBM both in the periphery and at the site of tissue damage was directed towards self-antigens. Peripheral autoantibodies present in IBM serum but not control serum recognized self-antigens in both muscle tissue and human-derived cell lines. To study the humoral immune response at the site of tissue damage in IBM patients, we isolated single plasma cells directly from IBM-derived muscle tissue sections and from these cells, reconstructed a series of recombinant immunoglobulins (rIgG). These rIgG, each representing a single muscle-associated plasma cell, were examined for reactivity to self-antigens. Both, flow cytometry and immunoblotting revealed that these rIgG recognized antigens expressed by cell lines and in muscle tissue homogenates. Using a mass spectrometry-based approach, Desmin, a major intermediate filament protein, expressed abundantly in muscle tissue, was identified as the target of one IBM muscle-derived rIgG. Collectively, these data support the view that IBM includes a humoral immune response in both the periphery and at the site of tissue damage that is directed towards self-antigens.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0046709en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465259/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectImmunologyen_US
dc.subjectImmunityen_US
dc.subjectHumoral Immunityen_US
dc.subjectAutoimmunityen_US
dc.subjectImmunoglobulinsen_US
dc.subjectImmunologic Techniquesen_US
dc.subjectImmunopathologyen_US
dc.subjectProteomicsen_US
dc.subjectSpectrometric Identification of Proteinsen_US
dc.subjectMedicineen_US
dc.subjectClinical Immunologyen_US
dc.subjectAutoimmune Diseasesen_US
dc.subjectNeurologyen_US
dc.subjectNeuromuscular Diseasesen_US
dc.titleAutoantibodies Produced at the Site of Tissue Damage Provide Evidence of Humoral Autoimmunity in Inclusion Body Myositisen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorAmato, Anthony A.
dc.date.available2013-04-10T15:04:05Z
dc.identifier.doi10.1371/journal.pone.0046709*
dash.authorsorderedfalse
dash.contributor.affiliatedAmato, Anthony
dash.contributor.affiliatedBradshaw, Elizabeth M.


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