A Portable Chemotaxis Platform for Short and Long Term Analysis

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A Portable Chemotaxis Platform for Short and Long Term Analysis

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Title: A Portable Chemotaxis Platform for Short and Long Term Analysis
Author: Xu, Chenjie; Poh, Yuk Kee C.; Roes, Isaac; O'Cearbhaill, Eoin D.; Matthiesen, Mads Emil; Mu, Luye; Yang, Seung Yun; Miranda-Nieves, David; Irimia, Daniel; Karp, Jeffrey Michael

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Citation: Xu, Chenjie, Yuk Kee C. Poh, Isaac Roes, Eoin D. O'Cearbhaill, Mads Emil Matthiesen, Luye Mu, Seung Yun Yang, David Miranda-Nieves, Daniel Irimia, and Jeffrey M. Karp. 2012. A portable chemotaxis platform for short and long term analysis. PLoS ONE 7(9): e44995.
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Abstract: Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells.
Published Version: doi:10.1371/journal.pone.0044995
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444504/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10524336
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