A Portable Chemotaxis Platform for Short and Long Term Analysis
Poh, Yuk Kee C.
O'Cearbhaill, Eoin D.
Matthiesen, Mads Emil
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CitationXu, Chenjie, Yuk Kee C. Poh, Isaac Roes, Eoin D. O'Cearbhaill, Mads Emil Matthiesen, Luye Mu, Seung Yun Yang, David Miranda-Nieves, Daniel Irimia, and Jeffrey M. Karp. 2012. A portable chemotaxis platform for short and long term analysis. PLoS ONE 7(9): e44995.
AbstractFlow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells.
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