Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence

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Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence

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Title: Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence
Author: Li, Chunqiang; Pastila, Riikka; Pitsillides, Costas; Runnels, Judith M.; Puoris’haag, Mehron; Côté, Daniel; Lin, Charles P.

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Citation: Li, Chunqiang, Riikka K. Pastila, Costas Pitsillides, Judith M. Runnels, Mehron Puoris’haag, Daniel Côté, and Charles P. Lin. 2010. Imaging leukocyte trafficking in vivo with two-photon-excited endogenous tryptophan fluorescence. Optics Express 18(2): 988-999.
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Abstract: We describe a new method for imaging leukocytes in vivo by exciting the endogenous protein fluorescence in the ultraviolet (UV) spectral region where tryptophan is the major fluorophore. Two-photon excitation near 590 nm allows noninvasive optical sectioning through the epidermal cell layers into the dermis of mouse skin, where leukocytes can be observed by video-rate microscopy to interact dynamically with the dermal vascular endothelium. Inflammation significantly enhances leukocyte rolling, adhesion, and tissue infiltration. After exiting the vasculature, leukocytes continue to move actively in tissue as observed by time-lapse microscopy, and are distinguishable from resident autofluorescent cells that are not motile. Because the new method alleviates the need to introduce exogenous labels, it is potentially applicable for tracking leukocytes and monitoring inflammatory cellular reactions in humans.
Published Version: doi:10.1364/OE.18.000988
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369551/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10524362
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