Graft versus Host Disease in the Bone Marrow, Liver and Thymus Humanized Mouse Model

DSpace/Manakin Repository

Graft versus Host Disease in the Bone Marrow, Liver and Thymus Humanized Mouse Model

Citable link to this page

 

 
Title: Graft versus Host Disease in the Bone Marrow, Liver and Thymus Humanized Mouse Model
Author: Vbranac, Vladimir; Tivey, Trevor; Greenblatt, Matthew Blake; Tager, Andrew Martin; Aliprantis, Antonios O.; Tsang, Kelly

Note: Order does not necessarily reflect citation order of authors.

Citation: Greenblatt, Matthew B., Vladimir Vbranac, Trevor Tivey, Kelly Tsang, Andrew M. Tager, and Antonios O. Aliprantis. 2012. Graft versus host disease in the bone marrow, liver and thymus humanized mouse model. PLoS ONE 7(9): e44664.
Full Text & Related Files:
Abstract: Mice bearing a “humanized” immune system are valuable tools to experimentally manipulate human cells in vivo and facilitate disease models not normally possible in laboratory animals. Here we describe a form of GVHD that develops in NOD/SCID mice reconstituted with human fetal bone marrow, liver and thymus (NS BLT mice). The skin, lungs, gastrointestinal tract and parotid glands are affected with progressive inflammation and sclerosis. Although all mice showed involvement of at least one organ site, the incidence of overt clinical disease was approximately 35% by 22 weeks after reconstitution. The use of hosts lacking the IL2 common gamma chain (NOD/SCID/γc−/−) delayed the onset of disease, but ultimately did not affect incidence. Genetic analysis revealed that particular donor HLA class I alleles influenced the risk for the development of GVHD. At a cellular level, GVHD is associated with the infiltration of human CD4+ T cells into the skin and a shift towards Th1 cytokine production. GVHD also induced a mixed M1/M2 polarization phenotype in a dermal murine CD11b+, MHC class II+ macrophage population. The presence of xenogenic GVHD in BLT mice both presents a major obstacle in the use of humanized mice and an opportunity to conduct preclinical studies on GVHD in a humanized model.
Published Version: doi:10.1371/journal.pone.0044664
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434179/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10533603
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters