In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology

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In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology

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Title: In Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technology
Author: Benink, Hélène A; McDougall, Mark G; Kung, Andrew L; Tseng, Jen-Chieh; Chico-Calero, Isabel Mariah

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Citation: Tseng, Jen-Chieh, Hélène A. Benink, Mark G. McDougall, Isabel Chico-Calero, and Andrew L. Kung. 2012. In vivo fluorescent labeling of tumor cells with the HaloTag® technology. Current Chemical Genomics 6(Suppl 1-M6): 48-54.
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Abstract: Many fluorescent sensors are currently available for in vitro bio-physiological microscopic imaging. The ability to label cells in living animals with these fluorescent sensors would help translate some of these assays into in vivo applications. To achieve this goal, the first step is to establish a method for selectively labeling target cells with exogenous fluorophores. Here we tested whether the HaloTag® protein tagging system provides specific labeling of xenograft tumors in living animals. After systemic delivery of fluorophore-conjugated ligands, we performed whole animal planar fluorescent imaging to determine uptake in tag-expressing HCT116 xenografts. Our results demonstrate that HaloTag ligands containing red or near-infrared fluorophores have enhanced tumor uptake and are suitable for non-invasive in vivo imaging. Our proof-of-concept results establish feasibility for using HaloTag technology for bio-physiological imaging in living animals.
Published Version: doi:10.2174/1875397301206010048
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480697/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10579114
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