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dc.contributor.authorTseng, Jen-Chieh
dc.contributor.authorBenink, Hélène A
dc.contributor.authorMcDougall, Mark G
dc.contributor.authorChico-Calero, Isabel Mariah
dc.contributor.authorKung, Andrew L
dc.date.accessioned2013-04-22T14:08:22Z
dc.date.issued2012
dc.identifier.citationTseng, Jen-Chieh, Hélène A. Benink, Mark G. McDougall, Isabel Chico-Calero, and Andrew L. Kung. 2012. In vivo fluorescent labeling of tumor cells with the HaloTag® technology. Current Chemical Genomics 6(Suppl 1-M6): 48-54.en_US
dc.identifier.issn1875-3973en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10579114
dc.description.abstractMany fluorescent sensors are currently available for in vitro bio-physiological microscopic imaging. The ability to label cells in living animals with these fluorescent sensors would help translate some of these assays into in vivo applications. To achieve this goal, the first step is to establish a method for selectively labeling target cells with exogenous fluorophores. Here we tested whether the HaloTag® protein tagging system provides specific labeling of xenograft tumors in living animals. After systemic delivery of fluorophore-conjugated ligands, we performed whole animal planar fluorescent imaging to determine uptake in tag-expressing HCT116 xenografts. Our results demonstrate that HaloTag ligands containing red or near-infrared fluorophores have enhanced tumor uptake and are suitable for non-invasive in vivo imaging. Our proof-of-concept results establish feasibility for using HaloTag technology for bio-physiological imaging in living animals.en_US
dc.language.isoen_USen_US
dc.publisherBentham Openen_US
dc.relation.isversionofdoi:10.2174/1875397301206010048en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480697/pdf/en_US
dash.licenseLAA
dc.subjectFluorescent imagingen_US
dc.subjectHaloTagen_US
dc.subjecttumor xenograften_US
dc.titleIn Vivo Fluorescent Labeling of Tumor Cells with the HaloTag® Technologyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalCurrent Chemical Genomicsen_US
dash.depositing.authorTseng, Jen-Chieh
dc.date.available2013-04-22T14:08:22Z
dc.identifier.doi10.2174/1875397301206010048*
dash.contributor.affiliatedChico-Calero, Isabel
dash.contributor.affiliatedTseng, Jen-Chieh


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