Targeting a Pre-mRNA Structure with Bipartite Antisense Molecules Modulates Tau Alternative Splicing

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Targeting a Pre-mRNA Structure with Bipartite Antisense Molecules Modulates Tau Alternative Splicing

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Title: Targeting a Pre-mRNA Structure with Bipartite Antisense Molecules Modulates Tau Alternative Splicing
Author: Peacey, Eleanor; Rodriguez, Lilia; Liu, Yang; Wolfe, Michael S.

Note: Order does not necessarily reflect citation order of authors.

Citation: Peacey, Eleanor, Lilia Rodriguez, Yang Liu, and Michael S. Wolfe. 2012. Targeting a pre-mRNA structure with bipartite antisense molecules modulates tau alternative splicing. Nucleic Acids Research 40(19): 9836-9849.
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Abstract: Approximately 15% of human genetic diseases are estimated to involve dysregulation of alternative pre-mRNA splicing. Antisense molecules designed to alter these and other splicing events typically target continuous linear sequences of the message. Here, we show that a structural feature in a pre-mRNA can be targeted by bipartite antisense molecules designed to hybridize with the discontinuous elements that flank the structure and thereby alter splicing. We targeted a hairpin structure at the boundary between exon 10 and intron 10 of the pre-mRNA of tau. Mutations in this region that are associated with certain forms of frontotemporal dementia, destabilize the hairpin to cause increased inclusion of exon 10. Via electrophoretic mobility shift and RNase protection assays, we demonstrate that bipartite antisense molecules designed to simultaneously interact with the available sequences that immediately flank the tau pre-mRNA hairpin do indeed bind to this structured region. Moreover, these agents inhibit exon 10 splicing and reverse the effect of destabilizing disease-causing mutations, in both in vitro splicing assays and cell culture. This general bipartite antisense strategy could be employed to modulate other splicing events that are regulated by RNA secondary structure.
Published Version: doi:10.1093/nar/gks710
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3479178/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10579120
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