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dc.contributor.authorKiwelu, Ireen E.
dc.contributor.authorNovitsky, Vladimir A.
dc.contributor.authorMargolin, Lauren
dc.contributor.authorBaca, Jeannie
dc.contributor.authorManongi, Rachel
dc.contributor.authorSam, Noel
dc.contributor.authorShao, John
dc.contributor.authorMcLane, Mary Frances
dc.contributor.authorKapiga, Saidi Hussein
dc.contributor.authorEssex, Myron Elmer
dc.date.accessioned2013-04-23T17:32:06Z
dc.date.issued2012
dc.identifier.citationKiwelu, Ireen E., Vladimir Novitsky, Lauren Margolin, Jeannie Baca, Rachel Manongi, Noel Sam, John Shao, Mary F. McLane, Saidi H. Kapiga, and M. Essex. 2012. HIV-1 subtypes and recombinants in Northern Tanzania: distribution of viral quasispecies. PLoS ONE 7(10): e47605.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10579564
dc.description.abstractThis study analyzed the distribution and prevalence of HIV-1 subtypes, multiplicity of HIV-1 infection, and frequency of inter-subtype recombination among HIV-1-infected female bar and hotel workers in Moshi, Kilimanjaro Region, Tanzania, from 2004 to 2007. The HIV-1 viral sequences spanning the V1-C5 region of HIV-1 env gp120 were analyzed from 50 subjects by single genome amplification and sequencing (SGA/S) technique. A total of 1740 sequences were amplified and sequenced from the HIV-1 proviral DNA template. The median env sequences analyzed per subject per two time points was 38 (IQR 28–50) over one year of HIV infection. In a subset of 14 subjects, a total of 239 sequences were obtained from HIV-1 RNA template at the baseline visit. The most prevalent HIV-1 subtypes were A1 (56%) and C (30%), while HIV-1 subtype D and inter-subtype recombinant viruses were found in 6% and 8% of subjects respectively. Transmission of multiple HIV-1 variants was evident in 27% of the subjects infected with pure HIV-1 subtypes A1, C, or D. The HIV-1 inter-subtype recombinants were found in 8% including HIV-1 C/A, D/A, and complex mosaic recombinants. Multiple viral variants were found in two subjects infected with inter-subtype recombinants. One subject harbored quasispecies of both pure HIV-1 A1 and C/A recombinant. The other subject was infected with two complex mosaic inter-subtype recombinant variants belonging to subtype D. HIV-1 multiple infections and ongoing recombination contribute significantly to the genetic diversity of circulating HIV-1 in Tanzania and have important implications for vaccine design and the development of therapeutic strategies.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0047605en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485255/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectComputational Biologyen_US
dc.subjectMolecular Geneticsen_US
dc.subjectMicrobiologyen_US
dc.subjectVirologyen_US
dc.subjectViral Classificationen_US
dc.subjectViral Transmission and Infectionen_US
dc.subjectPopulation Biologyen_US
dc.subjectEpidemiologyen_US
dc.subjectInfectious Disease Epidemiologyen_US
dc.subjectMedicineen_US
dc.subjectInfectious Diseasesen_US
dc.subjectViral Diseasesen_US
dc.subjectHIVen_US
dc.subjectHIV epidemiologyen_US
dc.titleHIV-1 Subtypes and Recombinants in Northern Tanzania: Distribution of Viral Quasispeciesen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorEssex, Myron Elmer
dc.date.available2013-04-23T17:32:06Z
dc.identifier.doi10.1371/journal.pone.0047605*
dash.contributor.affiliatedKapiga, Saidi Hussein
dash.contributor.affiliatedMcLane, Mary
dash.contributor.affiliatedEssex, Myron
dash.contributor.affiliatedNovitsky, Vladimir


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