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dc.contributor.authorJi, Jun-Yuan
dc.contributor.authorMiles, Wayne O.
dc.contributor.authorKorenjak, Michael
dc.contributor.authorZheng, Yani
dc.contributor.authorDyson, Nicholas J.
dc.date.accessioned2013-04-24T20:39:17Z
dc.date.issued2012
dc.identifier.citationJi, Jun-Yuan, Wayne O. Miles, Michael Korenjak, Yani Zheng, and Nicholas J. Dyson. 2012. In vivo regulation of E2F1 by polycomb group genes in drosophila. G3: Genes|Genomes|Genetics 2(12): 1651-1660.en_US
dc.identifier.issn2160-1836en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10582101
dc.description.abstractThe E2F transcription factors are important regulators of the cell cycle whose function is commonly misregulated in cancer. To identify novel regulators of E2F1 activity in vivo, we used Drosophila to conduct genetic screens. For this, we generated transgenic lines that allow the tissue-specific depletion of dE2F1 by RNAi. Expression of these transgenes using Gal4 drivers in the eyes and wings generated reliable and modifiable phenotypes. We then conducted genetic screens testing the capacity of Exelixis deficiencies to modify these E2F1-RNAi phenotypes. From these screens, we identified mutant alleles of Suppressor of zeste 2 [Su(z)2] and multiple Polycomb group genes as strong suppressors of the E2F1-RNA interference phenotypes. In validation of our genetic data, we find that depleting Su(z)2 in cultured Drosophila cells restores the cell-proliferation defects caused by reduction of dE2F1 by elevating the level of dE2f1. Furthermore, analyses of methylation status of histone H3 lysine 27 (H3K27me) from the published modENCODE data sets suggest that the genomic regions harboring dE2f1 gene and certain dE2f1 target genes display H3K27me during development and in several Drosophila cell lines. These in vivo observations suggest that the Polycomb group may regulate cell proliferation by repressing the transcription of dE2f1 and certain dE2F1 target genes. This mechanism may play an important role in coordinating cellular differentiation and proliferation during Drosophila development.en_US
dc.language.isoen_USen_US
dc.publisherGenetics Society of Americaen_US
dc.relation.isversionofdoi:10.1534/g3.112.004333en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516486/pdf/en_US
dash.licenseLAA
dc.subjectcell proliferationen_US
dc.subjectE2F1en_US
dc.subjectSu(z)2en_US
dc.subjectPcGen_US
dc.titleIn Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophilaen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalG3: Genes|Genomes|Geneticsen_US
dash.depositing.authorMiles, Wayne O.
dc.date.available2013-04-24T20:39:17Z
dc.identifier.doi10.1534/g3.112.004333*
dash.contributor.affiliatedMiles, Wayne O.
dash.contributor.affiliatedKorenjak, Michael
dash.contributor.affiliatedDyson, Nicholas


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