Show simple item record

dc.contributor.authorSeow, Wei Jie
dc.contributor.authorPesatori, Angela Cecilia
dc.contributor.authorDimont, Emmanuel
dc.contributor.authorFarmer, Peter B.
dc.contributor.authorAlbetti, Benedetta
dc.contributor.authorEttinger, Adrienne S.
dc.contributor.authorBollati, Valentina
dc.contributor.authorBolognesi, Claudia
dc.contributor.authorRoggieri, Paola
dc.contributor.authorPanev, Teodor I.
dc.contributor.authorGeorgieva, Tzveta
dc.contributor.authorMerlo, Domenico Franco
dc.contributor.authorBertazzi, Pier Alberto
dc.contributor.authorBaccarelli, Andrea
dc.date.accessioned2013-04-25T18:10:56Z
dc.date.issued2012
dc.identifier.citationSeow, Wei Jie, Angela Cecilia Pesatori, Emmanuel Dimont, Peter B. Farmer, Benedetta Albetti, Adrienne S. Ettinger, Valentina Bollati, et al. 2012. Urinary benzene biomarkers and DNA methylation in Bulgarian petrochemical workers: study findings and comparison of linear and beta regression models. PLoS ONE 7(12): e50471.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10587967
dc.description.abstractChronic occupational exposure to benzene is associated with an increased risk of hematological malignancies such as acute myeloid leukemia (AML), but the underlying mechanisms are still unclear. The main objective of this study was to investigate the association between benzene exposure and DNA methylation, both in repeated elements and candidate genes, in a population of 158 Bulgarian petrochemical workers and 50 unexposed office workers. Exposure assessment included personal monitoring of airborne benzene at work and urinary biomarkers of benzene metabolism (S-phenylmercapturic acid [SPMA] and trans,trans-muconic acid [t,t-MA]) at the end of the work-shift. The median levels of airborne benzene, SPMA and t,t-MA in workers were 0.46 ppm, 15.5 µg/L and 711 µg/L respectively, and exposure levels were significantly lower in the controls. Repeated-element DNA methylation was measured in Alu and LINE-1, and gene-specific methylation in MAGE and p15. DNA methylation levels were not significantly different between exposed workers and controls (P>0.05). Both ordinary least squares (OLS) and beta-regression models were used to estimate benzene-methylation associations. Beta-regression showed better model specification, as reflected in improved coefficient of determination (pseudo \(R^2\)) and Akaike’s information criterion (AIC). In beta-regression, we found statistically significant reductions in LINE-1 (−0.15%, P<0.01) and p15 (−0.096%, P<0.01) mean methylation levels with each interquartile range (IQR) increase in SPMA. This study showed statistically significant but weak associations of LINE-1 and p15 hypomethylation with SPMA in Bulgarian petrochemical workers. We showed that beta-regression is more appropriate than OLS regression for fitting methylation data.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0050471en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515615/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectGeneticsen_US
dc.subjectEpigeneticsen_US
dc.subjectPopulation Biologyen_US
dc.subjectEpidemiologyen_US
dc.subjectEnvironmental Epidemiologyen_US
dc.subjectEpidemiological Methodsen_US
dc.subjectMathematicsen_US
dc.subjectStatisticsen_US
dc.subjectBiostatisticsen_US
dc.subjectMedicineen_US
dc.subjectClinical Research Designen_US
dc.subjectStatistical Methodsen_US
dc.subjectBiomarker Epidemiologyen_US
dc.subjectCancer Epidemiologyen_US
dc.subjectMolecular Epidemiologyen_US
dc.subjectPublic Healthen_US
dc.subjectOccupational and Industrial Healthen_US
dc.subjectToxicologyen_US
dc.subjectGenetic Toxicologyen_US
dc.titleUrinary Benzene Biomarkers and DNA Methylation in Bulgarian Petrochemical Workers: Study Findings and Comparison of Linear and Beta Regression Modelsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorBaccarelli, Andrea
dc.date.available2013-04-25T18:10:56Z
dc.identifier.doi10.1371/journal.pone.0050471*
dash.authorsorderedfalse
dash.contributor.affiliatedDimont, Emmanuel
dash.contributor.affiliatedSeow, Wei Jie
dash.contributor.affiliatedEttinger, Adrienne
dash.contributor.affiliatedBaccarelli, Andrea
dc.identifier.orcid0000-0002-3436-0640


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record