Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis
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Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis
| Title: | Global Assessment of Genomic Regions Required for Growth in Mycobacterium tuberculosis |
| Author: |
Ioerger, Thomas R.; Long, Jarukit E.; Sassetti, Christopher M.; Sacchettini, James C.; Huttenhower, Curtis; Rubin, Eric Joseph; Zhang, Yanjia Jason
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Zhang, Yanjia J., Thomas R. Ioerger, Curtis Huttenhower, Jarukit E. Long, Christopher M. Sassetti, James C. Sacchettini, and Eric J. Rubin. 2012. Global assessment of genomic regions required for growth in Mycobacterium tuberculosis. PLoS Pathogens 8(9): e1002946. |
| Full Text & Related Files: |
3460630.pdf (2.534Mb; PDF) 
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| Abstract: | Identifying genomic elements required for viability is central to our understanding of the basic physiology of bacterial pathogens. Recently, the combination of high-density mutagenesis and deep sequencing has allowed for the identification of required and conditionally required genes in many bacteria. Genes, however, make up only a part of the complex genomes of important bacterial pathogens. Here, we use an unbiased analysis to comprehensively identify genomic regions, including genes, domains, and intergenic elements, required for the optimal growth of Mycobacterium tuberculosis, a major global health pathogen. We found that several proteins jointly contain both domains required for optimal growth and domains that are dispensable. In addition, many non-coding regions, including regulatory elements and non-coding RNAs, are critical for mycobacterial growth. Our analysis shows that the genetic requirements for growth are more complex than can be appreciated using gene-centric analysis. |
| Published Version: | doi:10.1371/journal.ppat.1002946 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460630/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10587988 |
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