RUNX1 Reshapes the Epigenetic Landscape at the Onset of Haematopoiesis

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RUNX1 Reshapes the Epigenetic Landscape at the Onset of Haematopoiesis

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Title: RUNX1 Reshapes the Epigenetic Landscape at the Onset of Haematopoiesis
Author: Lichtinger, Monika; Ingram, Richard; Hannah, Rebecca; Müller, Dorothee; Clarke, Deborah; Assi, Salam A; Lie-A-Ling, Michael; Noailles, Laura; Vijayabaskar, M S; Wu, Mengchu; Westhead, David R; Kouskoff, Valerie; Lacaud, Georges; Göttgens, Berthold; Bonifer, Constanze; Tenen, Daniel Geoffrey

Note: Order does not necessarily reflect citation order of authors.

Citation: Lichtinger, Monika, Richard Ingram, Rebecca Hannah, Dorothee Müller, Deborah Clarke, Salam A. Assi, Michael Lie-A-Ling, et al. 2012. RUNX1 reshapes the epigenetic landscape at the onset of haematopoiesis. The EMBO Journal 31(22): 4318-4333.
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Abstract: Cell fate decisions during haematopoiesis are governed by lineage-specific transcription factors, such as RUNX1, SCL/TAL1, FLI1 and C/EBP family members. To gain insight into how these transcription factors regulate the activation of haematopoietic genes during embryonic development, we measured the genome-wide dynamics of transcription factor assembly on their target genes during the RUNX1-dependent transition from haemogenic endothelium (HE) to haematopoietic progenitors. Using a \(Runx1^{−/−}\) embryonic stem cell differentiation model expressing an inducible Runx1 gene, we show that in the absence of RUNX1, haematopoietic genes bind SCL/TAL1, FLI1 and C/EBPβ and that this early priming is required for correct temporal expression of the myeloid master regulator PU.1 and its downstream targets. After induction, RUNX1 binds to numerous de novo sites, initiating a local increase in histone acetylation and rapid global alterations in the binding patterns of SCL/TAL1 and FLI1. The acquisition of haematopoietic fate controlled by Runx1 therefore does not represent the establishment of a new regulatory layer on top of a pre-existing HE program but instead entails global reorganization of lineage-specific transcription factor assemblies.
Published Version: doi:10.1038/emboj.2012.275
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501222/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10591703
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