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dc.contributor.authorSelimyan, Roza
dc.contributor.authorGerstein, Rachel M.
dc.contributor.authorIvanova, Irina
dc.contributor.authorPrecht, Patricia
dc.contributor.authorSubrahmanyam, Ramesh
dc.contributor.authorPerlot, Thomas
dc.contributor.authorAlt, Frederick W.
dc.contributor.authorSen, Ranjan
dc.date.accessioned2013-04-30T20:58:03Z
dc.date.issued2013
dc.identifier.citationSelimyan, Roza, Rachel M. Gerstein, Irina Ivanova, Patricia Precht, Ramesh Subrahmanyam, Thomas Perlot, Frederick W. Alt, and Ranjan Sen. 2013. Localized dna demethylation at recombination intermediates during immunoglobulin heavy chain gene assembly. PLoS Biology 11(1): e1001475.en_US
dc.identifier.issn1544-9173en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10594431
dc.description.abstractMultiple epigenetic marks have been proposed to contribute to the regulation of antigen receptor gene assembly via V(D)J recombination. Here we provide a comprehensive view of DNA methylation at the immunoglobulin heavy chain (IgH) gene locus prior to and during V(D)J recombination. DNA methylation did not correlate with the histone modification state on unrearranged alleles, indicating that these epigenetic marks were regulated independently. Instead, pockets of tissue-specific demethylation were restricted to DNase I hypersensitive sites within this locus. Though unrearranged diversity (DH) and joining (JH) gene segments were methylated, DJH junctions created after the first recombination step were largely demethylated in pro-, pre-, and mature B cells. Junctional demethylation was highly localized, B-lineage-specific, and required an intact tissue-specific enhancer, Eμ. We propose that demethylation occurs after the first recombination step and may mark the junction for secondary recombination.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pbio.1001475en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558432/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.titleLocalized DNA Demethylation at Recombination Intermediates during Immunoglobulin Heavy Chain Gene Assemblyen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Biologyen_US
dash.depositing.authorAlt, Frederick W.
dc.date.available2013-04-30T20:58:03Z
dc.identifier.doi10.1371/journal.pbio.1001475*
dash.contributor.affiliatedAlt, Frederick


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