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dc.contributor.authorMcCoy, Laura E.
dc.contributor.authorQuigley, Anna Forsman
dc.contributor.authorStrokappe, Nika M.
dc.contributor.authorBulmer-Thomas, Bianca
dc.contributor.authorMortier, Daniella
dc.contributor.authorRutten, Lucy
dc.contributor.authorChander, Nikita
dc.contributor.authorEdwards, Carolyn J.
dc.contributor.authorKetteler, Robin
dc.contributor.authorVerrips, Theo
dc.contributor.authorWeiss, Robin A.
dc.contributor.authorSeaman, Michael S.
dc.contributor.authorDavis, David
dc.date.accessioned2013-05-03T13:10:01Z
dc.date.issued2012
dc.identifier.citationMcCoy, Laura E., Anna Forsman Quigley, Nika M. Strokappe, Bianca Bulmer-Thomas, Michael S. Seaman, Daniella Mortier, Lucy Rutten, et al. 2012. Potent and broad neutralization of HIV-1 by a llama antibody elicited by immunization. The Journal of Experimental Medicine 209(6): 1091-1103.en_US
dc.identifier.issn0022-1007en_US
dc.identifier.issn1540-9538en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10610337
dc.description.abstractLlamas (Lama glama) naturally produce heavy chain–only antibodies (Abs) in addition to conventional Abs. The variable regions (VHH) in these heavy chain–only Abs demonstrate comparable affinity and specificity for antigens to conventional immunoglobulins despite their much smaller size. To date, immunizations in humans and animal models have yielded only Abs with limited ability to neutralize HIV-1. In this study, a VHH phagemid library generated from a llama that was multiply immunized with recombinant trimeric HIV-1 envelope proteins (Envs) was screened directly for HIV-1 neutralization. One VHH, L8CJ3 (J3), neutralized 96 of 100 tested HIV-1 strains, encompassing subtypes A, B, C, D, BC, AE, AG, AC, ACD, CD, and G. J3 also potently neutralized chimeric simian-HIV strains with HIV subtypes B and C Env. The sequence of J3 is highly divergent from previous anti–HIV-1 VHH and its own germline sequence. J3 achieves broad and potent neutralization of HIV-1 via interaction with the CD4-binding site of HIV-1 Env. This study may represent a new benchmark for immunogens to be included in B cell–based vaccines and supports the development of VHH as anti–HIV-1 microbicides.en_US
dc.language.isoen_USen_US
dc.publisherThe Rockefeller University Pressen_US
dc.relation.isversionofdoi:10.1084/jem.20112655en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371729/pdf/en_US
dash.licenseLAA
dc.titlePotent and Broad Neutralization of HIV-1 by a Llama Antibody Elicited by Immunizationen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalThe Journal of Experimental Medicineen_US
dash.depositing.authorSeaman, Michael S.
dc.date.available2013-05-03T13:10:01Z
dc.identifier.doi10.1084/jem.20112655*
dash.authorsorderedfalse
dash.contributor.affiliatedSeaman, Michael


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