The IFT-A Complex Regulates Shh Signaling through Cilia Structure and Membrane Protein Trafficking

DSpace/Manakin Repository

The IFT-A Complex Regulates Shh Signaling through Cilia Structure and Membrane Protein Trafficking

Citable link to this page

 

 
Title: The IFT-A Complex Regulates Shh Signaling through Cilia Structure and Membrane Protein Trafficking
Author: Liem, Karel F., Jr.; Ashe, Alyson; He, Mu; Satir, Peter; Moran, Jennifer; Wicking, Carol; Anderson, Kathryn V.; Beier, David Randolph

Note: Order does not necessarily reflect citation order of authors.

Citation: Liem, Karel F., Jr., Alyson Ashe, Mu He, Peter Satir, Jennifer Moran, David Randolph Beier, Carol Wicking, and Kathryn V. Anderson. 2012. The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking. The Journal of Cell Biology 197(6): 789-800.
Full Text & Related Files:
Abstract: Two intraflagellar transport (IFT) complexes, IFT-A and IFT-B, build and maintain primary cilia and are required for activity of the Sonic hedgehog (Shh) pathway. A weak allele of the IFT-A gene, Ift144, caused subtle defects in cilia structure and ectopic activation of the Shh pathway. In contrast, strong loss of IFT-A, caused by either absence of Ift144 or mutations in two IFT-A genes, blocked normal ciliogenesis and decreased Shh signaling. In strong IFT-A mutants, the Shh pathway proteins Gli2, Sufu, and Kif7 localized correctly to cilia tips, suggesting that these pathway components were trafficked by IFT-B. In contrast, the membrane proteins Arl13b, ACIII, and Smo failed to localize to primary cilia in the absence of IFT-A. We propose that the increased Shh activity seen in partial loss-of-function IFT-A mutants may be a result of decreased ciliary ACIII and that the loss of Shh activity in the absence of IFT-A is a result of severe disruptions of cilia structure and membrane protein trafficking.
Published Version: doi:10.1083/jcb.201110049
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3373400/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10610353
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters