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dc.contributor.authorDjonović, Slavica
dc.contributor.authorUrbach, Jonathan M.
dc.contributor.authorDrenkard, Eliana Patricia
dc.contributor.authorBush, Jenifer
dc.contributor.authorFeinbaum, Rhonda Lorin
dc.contributor.authorAusubel, Jonathan Lee
dc.contributor.authorTraficante, David
dc.contributor.authorRisech, Martina
dc.contributor.authorKocks, Christine
dc.contributor.authorFischbach, Michael A.
dc.contributor.authorPriebe, Gregory Paul
dc.contributor.authorAusubel, Frederick M.
dc.date.accessioned2013-05-07T14:28:45Z
dc.date.issued2013
dc.identifier.citationDjonović, Slavica, Jonathan M. Urbach, Eliana Drenkard, Jenifer Bush, Rhonda Feinbaum, Jonathan L. Ausubel, David Traficante, et al. 2013. Trehalose biosynthesis promotes Pseudomonas aeruginosa pathogenicity in plants. PLoS Pathogens 9(3): e1003217.en_US
dc.identifier.issn1553-7366en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10611672
dc.description.abstractPseudomonas aeruginosa strain PA14 is a multi-host pathogen that infects plants, nematodes, insects, and vertebrates. Many PA14 factors are required for virulence in more than one of these hosts. Noting that plants have a fundamentally different cellular architecture from animals, we sought to identify PA14 factors that are specifically required for plant pathogenesis. We show that synthesis by PA14 of the disaccharide trehalose is required for pathogenesis in Arabidopsis, but not in nematodes, insects, or mice. In-frame deletion of two closely-linked predicted trehalose biosynthetic operons, treYZ and treS, decreased growth in Arabidopsis leaves about 50 fold. Exogenously co-inoculated trehalose, ammonium, or nitrate, but not glucose, sulfate, or phosphate suppressed the phenotype of the double ΔtreYZΔtreS mutant. Exogenous trehalose or ammonium nitrate does not suppress the growth defect of the double ΔtreYZΔtreS mutant by suppressing the plant defense response. Trehalose also does not function intracellularly in P. aeruginosa to ameliorate a variety of stresses, but most likely functions extracellularly, because wild-type PA14 rescued the in vivo growth defect of the ΔtreYZΔtreS in trans. Surprisingly, the growth defect of the double ΔtreYZΔtreS double mutant was suppressed by various Arabidopsis cell wall mutants that affect xyloglucan synthesis, including an xxt1xxt2 double mutant that completely lacks xyloglucan, even though xyloglucan mutants are not more susceptible to pathogens and respond like wild-type plants to immune elicitors. An explanation of our data is that trehalose functions to promote the acquisition of nitrogen-containing nutrients in a process that involves the xyloglucan component of the plant cell wall, thereby allowing P. aeruginosa to replicate in the intercellular spaces in a leaf. This work shows how P. aeruginosa, a multi-host opportunistic pathogen, has repurposed a highly conserved “house-keeping” anabolic pathway (trehalose biosynthesis) as a potent virulence factor that allows it to replicate in the intercellular environment of a leaf.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.ppat.1003217en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3591346/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectGeneticsen_US
dc.subjectGenomicsen_US
dc.subjectMicrobiologyen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectPlant Scienceen_US
dc.titleTrehalose Biosynthesis Promotes Pseudomonas aeruginosa Pathogenicity in Plantsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS Pathogensen_US
dash.depositing.authorPriebe, Gregory Paul
dc.date.available2013-05-07T14:28:45Z
dc.identifier.doi10.1371/journal.ppat.1003217*
dash.authorsorderedfalse
dash.contributor.affiliatedDrenkard, Eliana
dash.contributor.affiliatedAusubel, Jonathan Lee
dash.contributor.affiliatedPriebe, Gregory
dash.contributor.affiliatedFeinbaum, Rhonda
dash.contributor.affiliatedAusubel, Frederick


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