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dc.contributor.authorCarvunis, Anne-Ruxandra
dc.contributor.authorRolland, Thomas
dc.contributor.authorWapinski, Ilan Neustat
dc.contributor.authorCalderwood, Michael A
dc.contributor.authorYildirim, Muhammed
dc.contributor.authorSimonis, Nicolas
dc.contributor.authorCharloteaux, Benoit
dc.contributor.authorHidalgo, César A.
dc.contributor.authorBarbette, Justin
dc.contributor.authorSanthanam, Balaji
dc.contributor.authorBrar, Gloria A.
dc.contributor.authorWeissman, Jonathan S.
dc.contributor.authorRegev, Aviv
dc.contributor.authorThierry-Mieg, Nicolas
dc.contributor.authorCusick, Michael
dc.contributor.authorVidal, Marc
dc.date.accessioned2013-05-07T16:03:34Z
dc.date.issued2012
dc.identifier.citationCarvunis, Anne-Ruxandra, Thomas Rolland, Ilan Wapinski, Michael A. Calderwood, Muhammed A. Yildirim, Nicolas Simonis, Benoit Charloteaux, et al. 2012. Proto-genes and de novo gene birth. Nature 487(7407): 370-374.en_US
dc.identifier.issn0028-0836en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10611736
dc.description.abstractNovel protein-coding genes can arise either through re-organization of pre-existing genes or de novo. Processes involving re-organization of pre-existing genes, notably following gene duplication, have been extensively described. In contrast, de novo gene birth remains poorly understood, mainly because translation of sequences devoid of genes, or “non-genic” sequences, is expected to produce insignificant polypeptides rather than proteins with specific biological functions. Here, we formalize an evolutionary model according to which functional genes evolve de novo through transitory proto-genes generated by widespread translational activity in non-genic sequences. Testing this model at genome-scale in Saccharomyces cerevisiae, we detect translation of hundreds of short species-specific open reading frames (ORFs) located in non-genic sequences. These translation events appear to provide adaptive potential, as suggested by their differential regulation upon stress and by signatures of retention by natural selection. In line with our model, we establish that S. cerevisiae ORFs can be placed within an evolutionary continuum ranging from non-genic sequences to genes. We identify ~1,900 candidate proto-genes among S. cerevisiae ORFs and find that de novo gene birth from such a reservoir may be more prevalent than sporadic gene duplication. Our work illustrates that evolution exploits seemingly dispensable sequences to generate adaptive functional innovation.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/nature11184en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3401362/pdf/en_US
dash.licenseLAA
dc.titleProto-genes and De Novo Gene Birthen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNatureen_US
dash.depositing.authorVidal, Marc
dc.date.available2013-05-07T16:03:34Z
dc.identifier.doi10.1038/nature11184*
dash.authorsorderedfalse
dash.contributor.affiliatedWapinski, Ilan
dash.contributor.affiliatedCarvunis, Anne-Ruxandra
dash.contributor.affiliatedCusick, Michael
dash.contributor.affiliatedRolland, Thomas
dash.contributor.affiliatedCalderwood, Michael
dash.contributor.affiliatedVidal, Marc
dash.contributor.affiliatedYildirim, Muhammed


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