Gas2l3, a Novel Constriction Site-Associated Protein Whose Regulation Is Mediated by the \(APC/C^{Cdh1}\) Complex
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Pe’er, Tal
Lahmi, Roxane
Sharaby, Yaara
Chorni, Evelin
Noach, Meirav
Vecsler, Manuela
Zlotorynski, Eitan
Steen, Judith A.
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1371/journal.pone.0057532Metadata
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Pe’er, Tal, Roxane Lahmi, Yaara Sharaby, Evelin Chorni, Meirav Noach, Manuela Vecsler, Eitan Zlotorynski, Hanno Steen, Judith A. Steen, and Amit Tzur. 2013. Gas2l3, a novel constriction site-associated protein whose regulation is mediated by the \(APC/C^{Cdh1}\) complex. PLoS ONE 8(2): e57532.Abstract
Growth arrest-specific 2-like protein 3 (Gas2l3) was recently identified as an Actin/Tubulin cross-linker protein that regulates cytokinesis. Using cell-free systems from both frog eggs and human cells, we show that the Gas2l3 protein is targeted for ubiquitin-mediated proteolysis by the \(APC/C^{Cdh1}\) complex, but not by the \(APC/C^{Cdc20}\) complex, and is phosphorylated by Cdk1 in mitosis. Moreover, late in cytokinesis, Gas2l3 is exclusively localized to the constriction sites, which are the narrowest parts of the intercellular bridge connecting the two daughter cells. Overexpression of Gas2l3 specifically interferes with cell abscission, which is the final stage of cell division, when the cutting of the intercellular bridge at the constriction sites occurs. We therefore suggest that Gas2l3 is part of the cellular mechanism that terminates cell division.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585356/pdf/Terms of Use
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