dc.contributor.author | Silberman, Daniel | |
dc.contributor.author | Bucknum, Amanda | |
dc.contributor.author | Bartlett, Thomas | |
dc.contributor.author | Composto, Gabriella | |
dc.contributor.author | Kozlowski, Megan | |
dc.contributor.author | Walker, Amanda | |
dc.contributor.author | Werda, Amy | |
dc.contributor.author | Cua, Jackelyn | |
dc.contributor.author | Somerville, John E. | |
dc.contributor.author | Riggs, James E. | |
dc.contributor.author | Sharpe, Arlene Helen | |
dc.date.accessioned | 2013-05-08T14:45:31Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Silberman, Daniel, Amanda Bucknum, Thomas Bartlett, Gabriella Composto, Megan Kozlowski, Amanda Walker, Amy Werda, et al. 2012. CD28 ligation increases macrophage suppression of T cell proliferation. Cellular & Molecular Immunology 9(4): 341-349. | en_US |
dc.identifier.issn | 1672-7681 | en_US |
dc.identifier.issn | 2042-0226 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10612562 | |
dc.description.abstract | When compared to spleen or lymph node cells, resident peritoneal cavity cells respond poorly to T cell activation in vitro. The greater proportional representation of macrophages in this cell source has been shown to actively suppress the T cell response. Peritoneal macrophages exhibit an immature phenotype \((MHC Class II^{lo}, B7^{lo})\) that reduces their efficacy as antigen presenting cells. Furthermore, these cells readily express inducible nitric oxide synthase (iNOS), an enzyme that promotes T cell tolerance by catabolism of the limiting amino acid arginine. Here, we investigate the ability of exogenous T cell costimulation to recover the peritoneal T cell response. We show that CD28 ligation failed to recover the peritoneal T cell response and actually suppressed responses that had been recovered by inhibiting iNOS. As indicated by cytokine ELISpot and neutralizing mAb treatment, this “co-suppression” response was due to CD28 ligation increasing the number of IFNγ-secreting cells. Our results illustrate that cellular composition and cytokine milieu influence T cell costimulation biology. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | doi:10.1038/cmi.2012.13 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3389597/pdf/ | en_US |
dash.license | LAA | |
dc.subject | CD28 | en_US |
dc.subject | Costimulation | en_US |
dc.subject | Macrophages | en_US |
dc.subject | Suppression | en_US |
dc.title | CD28 Ligation Increases Macrophage Suppression of T Cell Proliferation | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Accepted Manuscript | en_US |
dc.relation.journal | Cellular & Molecular Immunology | en_US |
dash.depositing.author | Sharpe, Arlene Helen | |
dc.date.available | 2013-05-08T14:45:31Z | |
dc.identifier.doi | 10.1038/cmi.2012.13 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Sharpe, Arlene | |