A novel knee prosthesis model of implant-related osteo- myelitis in rats
Søe, Niels H
Jensen, Nina Vendel
Nürnberg, Birgit Meinecke
Jensen, Asger Lundorff
Poulsen, Steen Seier
Johansen, Helle KroghNote: Order does not necessarily reflect citation order of authors.
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CitationSøe, Niels H, Nina Vendel Jensen, Birgit Meinecke Nürnberg, Asger Lundorff Jensen, Janne Koch, Steen Seier Poulsen, Gerald Pier, and Helle Krogh Johansen. 2011. A novel knee prosthesis model of implant-related osteo- myelitis in rats. Acta Orthopaedica 84(1): 92-97.
AbstractBackground and purpose: There have been numerous reports of animal models of osteomyelitis. Very few of these have been prosthesis models that imitate human conditions. We have developed a new rat model of implant-related osteomyelitis that mimics human osteomyelitis, to investigate the pathology of infection after orthop edic implant surgery. Methods: 2 wild-type strains of Staphylococcus aureus, MN8 and UAMS-1, and their corresponding mutants that are unable to produce poly-N-acetyl glucosamine (PNAG) (ica::tet) were injected into the medullary canals of the femur and tibia at 3 different doses: \(10^2\), \(10^3\), and > \(10^4\) CFU/rat. We measured clinical signs, inflammatory markers, radiographic signs, histopathology, and bacteriology in the infected animals. Results: An inoculum of at least \(10^4\) cfu of either wild-type bacterial strain resulted in histological, bacteriological, and radiographic signs of osteomyelitis with loosening of the prosthesis. An inoculum of \(10^3\) CFU gave signs of osteomyelitis but the prosthesis remained in situ. Bacterial inocula of \(10^2\) cfu gave no signs of osteolysis. Interpretation: We have established a new knee prosthesis model that is suitable for reliable induction of experimental implant-associated osteomyelitis with the prosthesis in situ, using a small inoculum of S. aureus. At a dose of \(10^3\) CFU/rat, bacteria unable to produce PNAG (ica::tet) had only minor defects in their virulence.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10612575
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