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dc.contributor.authorChang, Jennifer S.
dc.contributor.authorWinston, Fred Marshall
dc.date.accessioned2013-05-08T19:04:48Z
dc.date.issued2013
dc.identifier.citationChang, Jennifer S., and Fred Winston. 2013. Cell-cycle perturbations suppress the slow-growth defect of spt10Δ mutants in saccharomyces cerevisiae. G3: Genes|Genomes|Genetics 3(3): 573-583.en_US
dc.identifier.issn2160-1836en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10612852
dc.description.abstractSpt10 is a putative acetyltransferase of Saccharomyces cerevisiae that directly activates the transcription of histone genes. Deletion of SPT10 causes a severe slow growth phenotype, showing that Spt10 is critical for normal cell division. To gain insight into the function of Spt10, we identified mutations that impair or improve the growth of spt10 null (spt10Δ) mutants. Mutations that cause lethality in combination with spt10Δ include particular components of the SAGA complex as well as asf1Δ and hir1Δ. Partial suppressors of the spt10Δ growth defect include mutations that perturb cell-cycle progression through the G1/S transition, S phase, and G2/M. Consistent with these results, slowing of cell-cycle progression by treatment with hydroxyurea or growth on medium containing glycerol as the carbon source also partially suppresses the spt10Δ slow-growth defect. In addition, mutations that impair the Lsm1-7−Pat1 complex, which regulates decapping of polyadenylated mRNAs, also partially suppress the spt10Δ growth defect. Interestingly, suppression of the spt10Δ growth defect is not accompanied by a restoration of normal histone mRNA levels. These findings suggest that Spt10 has multiple roles during cell division.en_US
dc.language.isoen_USen_US
dc.publisherGenetics Society of Americaen_US
dc.relation.isversionofdoi:10.1534/g3.112.005389en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583463/pdf/en_US
dash.licenseLAA
dc.subjectSpt10en_US
dc.subjectSpt21en_US
dc.subjecthistonesen_US
dc.subjectsuppressorsen_US
dc.titleCell-Cycle Perturbations Suppress the Slow-Growth Defect of spt10Δ Mutants in Saccharomyces cerevisiaeen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalG3: Genes|Genomes|Geneticsen_US
dash.depositing.authorWinston, Fred Marshall
dc.date.available2013-05-08T19:04:48Z
dc.identifier.doi10.1534/g3.112.005389*
dash.contributor.affiliatedWinston, Fred


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