Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase

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Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase

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Title: Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase
Author: van Rijn, Sjoerd; Nilsson, Jonas; Noske, David P.; Vandertop, W. Peter; Tannous, Bakhos A.; Würdinger, Thomas

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Citation: Van Rijn, Sjoerd, Jonas Nilsson, David P. Noske, W. Peter Vandertop, Bakhos A. Tannous, and Thomas Würdinger. 2013. Functional multiplex reporter assay using tagged Gaussia luciferase. Scientific Reports 3: 1046.
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Abstract: We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc\(_{tag}\)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc\(_{tag}\) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.
Published Version: doi:10.1038/srep01046
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10622999
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