dc.contributor.author | van Rijn, Sjoerd | |
dc.contributor.author | Nilsson, Jonas | |
dc.contributor.author | Noske, David P. | |
dc.contributor.author | Vandertop, W. Peter | |
dc.contributor.author | Tannous, Bakhos A. | |
dc.contributor.author | Würdinger, Thomas | |
dc.date.accessioned | 2013-05-13T20:25:04Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Van Rijn, Sjoerd, Jonas Nilsson, David P. Noske, W. Peter Vandertop, Bakhos A. Tannous, and Thomas Würdinger. 2013. Functional multiplex reporter assay using tagged Gaussia luciferase. Scientific Reports 3: 1046. | en_US |
dc.identifier.issn | 2045-2322 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10622999 | |
dc.description.abstract | We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc\(_{tag}\)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc\(_{tag}\) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | doi:10.1038/srep01046 | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/pdf/ | en_US |
dash.license | LAA | |
dc.title | Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Scientific Reports | en_US |
dash.depositing.author | Tannous, Bakhos A. | |
dc.date.available | 2013-05-13T20:25:04Z | |
dc.identifier.doi | 10.1038/srep01046 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Tannous, Bakhos | |