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dc.contributor.authorLiu, Yong
dc.contributor.authorYang, Xuesen
dc.contributor.authorGuo, Chenying
dc.contributor.authorNie, Pan
dc.contributor.authorLiu, Yan
dc.contributor.authorMa, Jie
dc.date.accessioned2013-05-14T13:38:33Z
dc.date.issued2013
dc.identifier.citationLiu, Yong, Xuesen Yang, Chenying Guo, Pan Nie, Yan Liu, and Jie Ma. 2013. Essential role of MFG-E8 for phagocytic properties of microglial cells. PLOS ONE 8(2): e55754.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10628433
dc.description.abstractMilk fat globule factor-E8 (MFG-E8) has been regarded as a key factor involved in the phagocytosis of apoptotic cells. We induced a lentivirus into the microglial cells for the augmentation or abrogation of MFG-E8 expression in mouse microglial cells, and investigated phagocytosis of phosphatidylserine tagged human red blood cells (hRBCs) in co-cultures. Increased MFG-E8 levels were associated with a significant increase in phagocytic activity compared to the controls. Conversely, phagocytosis dramitically decreased due to the abrogation of MFG-E8. In addition, the expression of the inflammatory cytokines, TNF-α and IL-1β, also increased or decreased in the microglial cells with the augmentation or abrogation of MFG-E8, respectively. Our findings indicate that the enhanced expression of MFG-E8 could increase phagocytosis of apoptotic cells; conversely, the rate of phagocytosis and the expression of inflammatory cytokines decreased when MFG-E8 expression was knocked down. Our results confirm that MFG-E8 plays an important role in phagocytosis, and possibly serves as an essential signal molecule for microglial cells.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0055754en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565973/pdf/en_US
dash.licenseLAA
dc.subjectbiologyen_US
dc.subjectimmune physiologyen_US
dc.subjectcytokinesen_US
dc.subjectdevelopmental biologyen_US
dc.subjectmolecular developmenten_US
dc.subjectimmunologyen_US
dc.subjectimmune systemen_US
dc.subjectimmunityen_US
dc.subjectimmune activationen_US
dc.subjectimmune cellsen_US
dc.subjectimmune responseen_US
dc.subjectmodel organismsen_US
dc.subjectanimal modelsen_US
dc.subjectmouseen_US
dc.subjectmolecular cell biologyen_US
dc.subjectcell divisionen_US
dc.subjectcytokinesisen_US
dc.subjectmedicineen_US
dc.subjectclinical immunologyen_US
dc.subjectanatomyen_US
dc.subjectphysiologyen_US
dc.titleEssential Role of MFG-E8 for Phagocytic Properties of Microglial Cellsen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLOS ONEen_US
dash.depositing.authorGuo, Chenying
dc.date.available2013-05-14T13:38:33Z
dc.identifier.doi10.1371/journal.pone.0055754*
dash.authorsorderedfalse
dash.contributor.affiliatedGuo, Chenying
dash.contributor.affiliatedMa, Jie


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