Prolonged High Fat Diet Reduces Dopamine Reuptake without Altering DAT Gene Expression

DSpace/Manakin Repository

Prolonged High Fat Diet Reduces Dopamine Reuptake without Altering DAT Gene Expression

Citable link to this page


Title: Prolonged High Fat Diet Reduces Dopamine Reuptake without Altering DAT Gene Expression
Author: Cone, Jackson J.; Chartoff, Elena Halley; Potter, David N.; Ebner, Stephanie R.; Roitman, Mitchell F.

Note: Order does not necessarily reflect citation order of authors.

Citation: Cone, Jackson J., Elena H. Chartoff, David N. Potter, Stephanie R. Ebner, and Mitchell F. Roitman. 2013. Prolonged high fat diet reduces dopamine reuptake without altering dat gene expression. PLoS ONE 8(3): e58251.
Full Text & Related Files:
Abstract: The development of diet-induced obesity (DIO) can potently alter multiple aspects of dopamine signaling, including dopamine transporter (DAT) expression and dopamine reuptake. However, the time-course of diet-induced changes in DAT expression and function and whether such changes are dependent upon the development of DIO remains unresolved. Here, we fed rats a high (HFD) or low (LFD) fat diet for 2 or 6 weeks. Following diet exposure, rats were anesthetized with urethane and striatal DAT function was assessed by electrically stimulating the dopamine cell bodies in the ventral tegmental area (VTA) and recording resultant changes in dopamine concentration in the ventral striatum using fast-scan cyclic voltammetry. We also quantified the effect of HFD on membrane associated DAT in striatal cell fractions from a separate group of rats following exposure to the same diet protocol. Notably, none of our treatment groups differed in body weight. We found a deficit in the rate of dopamine reuptake in HFD rats relative to LFD rats after 6 but not 2 weeks of diet exposure. Additionally, the increase in evoked dopamine following a pharmacological challenge of cocaine was significantly attenuated in HFD relative to LFD rats. Western blot analysis revealed that there was no effect of diet on total DAT protein. However, 6 weeks of HFD exposure significantly reduced the 50 kDa DAT isoform in a synaptosomal membrane-associated fraction, but not in a fraction associated with recycling endosomes. Our data provide further evidence for diet-induced alterations in dopamine reuptake independent of changes in DAT production and demonstrates that such changes can manifest without the development of DIO.
Published Version: doi:10.1371/journal.pone.0058251
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search