Show simple item record

dc.contributor.authorCone, Jackson J.
dc.contributor.authorChartoff, Elena Halley
dc.contributor.authorPotter, David N.
dc.contributor.authorEbner, Stephanie R.
dc.contributor.authorRoitman, Mitchell F.
dc.date.accessioned2013-05-23T19:40:01Z
dc.date.issued2013
dc.identifier.citationCone, Jackson J., Elena H. Chartoff, David N. Potter, Stephanie R. Ebner, and Mitchell F. Roitman. 2013. Prolonged high fat diet reduces dopamine reuptake without altering dat gene expression. PLoS ONE 8(3): e58251.en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10658101
dc.description.abstractThe development of diet-induced obesity (DIO) can potently alter multiple aspects of dopamine signaling, including dopamine transporter (DAT) expression and dopamine reuptake. However, the time-course of diet-induced changes in DAT expression and function and whether such changes are dependent upon the development of DIO remains unresolved. Here, we fed rats a high (HFD) or low (LFD) fat diet for 2 or 6 weeks. Following diet exposure, rats were anesthetized with urethane and striatal DAT function was assessed by electrically stimulating the dopamine cell bodies in the ventral tegmental area (VTA) and recording resultant changes in dopamine concentration in the ventral striatum using fast-scan cyclic voltammetry. We also quantified the effect of HFD on membrane associated DAT in striatal cell fractions from a separate group of rats following exposure to the same diet protocol. Notably, none of our treatment groups differed in body weight. We found a deficit in the rate of dopamine reuptake in HFD rats relative to LFD rats after 6 but not 2 weeks of diet exposure. Additionally, the increase in evoked dopamine following a pharmacological challenge of cocaine was significantly attenuated in HFD relative to LFD rats. Western blot analysis revealed that there was no effect of diet on total DAT protein. However, 6 weeks of HFD exposure significantly reduced the 50 kDa DAT isoform in a synaptosomal membrane-associated fraction, but not in a fraction associated with recycling endosomes. Our data provide further evidence for diet-induced alterations in dopamine reuptake independent of changes in DAT production and demonstrates that such changes can manifest without the development of DIO.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofdoi:10.1371/journal.pone.0058251en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596409/pdf/en_US
dash.licenseLAA
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectNeurochemistryen_US
dc.subjectNeurochemicalsen_US
dc.subjectDopamineen_US
dc.subjectModel Organismsen_US
dc.subjectAnimal Modelsen_US
dc.subjectRaten_US
dc.subjectNeuroscienceen_US
dc.subjectMolecular Neuroscienceen_US
dc.subjectSignaling Pathwaysen_US
dc.subjectNeurophysiologyen_US
dc.subjectCentral Nervous Systemen_US
dc.subjectHomeostatic Mechanismsen_US
dc.subjectBehavioral Neuroscienceen_US
dc.subjectNeurobiology of Disease and Regenerationen_US
dc.subjectNeuropsychologyen_US
dc.subjectNeurotransmittersen_US
dc.subjectSystems Biologyen_US
dc.subjectMedicineen_US
dc.subjectNutritionen_US
dc.subjectObesityen_US
dc.titleProlonged High Fat Diet Reduces Dopamine Reuptake without Altering DAT Gene Expressionen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalPLoS ONEen_US
dash.depositing.authorChartoff, Elena Halley
dc.date.available2013-05-23T19:40:01Z
dc.identifier.doi10.1371/journal.pone.0058251*
dash.contributor.affiliatedChartoff, Elena


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record