Direct observation of mammalian cell growth and size regulation

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Direct observation of mammalian cell growth and size regulation

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Title: Direct observation of mammalian cell growth and size regulation
Author: Son, Sungmin; Tzur, Amit; Weng, Yaochung; Jorgensen, Paul D; Kim, Jisoo; Kirschner, Marc W.; Manalis, Scott R.

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Citation: Son, Sungmin, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W. Kirschner, and Scott R. Manalis. 2012. Direct observation of mammalian cell growth and size regulation. Nature Methods 9(9): 910-912.
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Abstract: We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1/S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
Published Version: doi:10.1038/nmeth.2133
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433595/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10804732
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