Direct observation of mammalian cell growth and size regulation
Manalis, Scott R.
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CitationSon, Sungmin, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W. Kirschner, and Scott R. Manalis. 2012. Direct observation of mammalian cell growth and size regulation. Nature Methods 9(9): 910-912.
AbstractWe introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1/S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10804732
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