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dc.contributor.authorShi, Peng
dc.contributor.authorScott, Mark A
dc.contributor.authorGhosh, Balaram
dc.contributor.authorWan, Dongpeng
dc.contributor.authorWissner-Gross, Zachary
dc.contributor.authorMazitschek, Ralph
dc.contributor.authorHaggarty, Stephen John
dc.contributor.authorYanik, Mehmet Fatih
dc.date.accessioned2013-07-12T14:33:53Z
dc.date.issued2011
dc.identifier.citationShi, Peng, Mark A. Scott, Balaram Ghosh, Dongpeng Wan, Zachary Wissner-Gross, Ralph Mazitschek, Stephen J. Haggarty, and Mehmet Fatih Yanik. 2011. Identification of small molecules that enhance synaptogenesis using synapse microarrays. Nature Communications 2: 510.en_US
dc.identifier.issn2041-1723en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10872799
dc.description.abstractSynaptic function is affected in many brain diseases and disorders. Technologies for large-scale synapse assays can facilitate identification of drug leads. Here we report a “synapse microarray” technology that enables ultra-sensitive, high-throughput, and quantitative screening of synaptogenesis. Our platform enables the induction of synaptic structures in regular arrays by precise positioning of non-neuronal cells expressing synaptic proteins, while allowing neurites to grow freely around these cells. The technology increases by tenfold the sensitivity of the traditional assays, and simultaneously decreases the time required to capture synaptogenic events by an order of magnitude. It is readily incorporated into multiwell formats compatible with industrial high-throughput screening platforms. Using this technology, we screened a chemical library and identified novel histone deacetylase inhibitors that improve neuroligin-1 induced synaptogenesis via modulating class-I histone deacetylases. We also found a structure-activity relationship for designing novel potent histone deacetylase inhibitors, which can be applied towards development of new therapeutics.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/ncomms1518en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3544154/pdf/en_US
dash.licenseLAA
dc.titleIdentification of Small Molecules that Enhance Synaptogenesis Using Synapse Microarraysen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Communicationsen_US
dash.depositing.authorHaggarty, Stephen John
dc.date.available2013-07-12T14:33:53Z
dc.identifier.doi10.1038/ncomms1518*
dash.contributor.affiliatedGhosh, Balaram
dash.contributor.affiliatedMazitschek, Ralph
dash.contributor.affiliatedHaggarty, Stephen


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