dc.contributor.author | Lu, Fake | |
dc.contributor.author | Ji, Minbiao | |
dc.contributor.author | Fu, Dan | |
dc.contributor.author | Ni, Xiaohui | |
dc.contributor.author | Freudiger, Christian Wilhelm | |
dc.contributor.author | Holtom, Gary | |
dc.contributor.author | Xie, Xiaoliang Sunney | |
dc.date.accessioned | 2013-07-16T19:37:27Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Lu, Fa-Ke, Minbiao Ji, Dan Fu, Xiaohui Ni, Christian W. Freudiger, Gary Holtom, and X. Sunney Xie. 2012. Multicolor stimulated Raman scattering microscopy. Molecular Physics 110(15-16): 1927-1932. | en_US |
dc.identifier.issn | 0026-8976 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:10875724 | |
dc.description.abstract | Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation with picosecond pulse excitation can only realize rapid chemical mapping with a single Raman band. Here we present a novel SRS microscopic technique using a grating-based pulse shaper for excitation and a grating-based spectrograph for detection to achieve simultaneous multicolor SRS imaging with high sensitivity and high acquisition speeds. In particular, we use a linear combination of the measured \(CH_2\) and \(CH_3\) stretching signals to map the distributions of protein and lipid contents simultaneously. | en_US |
dc.description.sponsorship | Chemistry and Chemical Biology | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Informa UK (Taylor & Francis) | en_US |
dc.relation.isversionof | doi:10.1080/00268976.2012.695028 | en_US |
dash.license | OAP | |
dc.subject | coherent Raman scattering | en_US |
dc.subject | stimulated Raman scattering | en_US |
dc.subject | multicolor | en_US |
dc.subject | grating | en_US |
dc.subject | pulse shaper | en_US |
dc.subject | lock-in | en_US |
dc.subject | polystyrene | en_US |
dc.subject | poly(methyl methacrylate) | en_US |
dc.subject | mouse skin | en_US |
dc.subject | protein | en_US |
dc.subject | lipid | en_US |
dc.title | Multicolor stimulated Raman scattering microscopy | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Accepted Manuscript | en_US |
dc.relation.journal | Molecular Physics | en_US |
dash.depositing.author | Xie, Xiaoliang Sunney | |
dc.date.available | 2013-07-16T19:37:27Z | |
dc.identifier.doi | 10.1080/00268976.2012.695028 | * |
dash.contributor.affiliated | Lu, Fake | |
dash.contributor.affiliated | Freudiger, Christian Wilhelm | |
dash.contributor.affiliated | Ji, Minbiao | |
dash.contributor.affiliated | Fu, Dan | |
dash.contributor.affiliated | Ni, Xiaohui | |
dash.contributor.affiliated | Xie, Xiaoliang | |