Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy

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Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy

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Title: Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy
Author: Fu, Dan; Lu, Fake; Zhang, Xu; Freudiger, Christian Wilhelm; Pernik, Douglas R.; Holtom, Gary; Xie, Xiaoliang Sunney

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Citation: Fu, Dan, Fa-Ke Lu, Xu Zhang, Christian Freudiger, Douglas R. Pernik, Gary Holtom, and Xiaoliang Sunney Xie. 2012. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. Journal of the American Chemical Society 134(8): 3623-3626.
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Abstract: Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations have been limited to single Raman band measurements. We present a novel modulation multiplexing approach that allows real-time detection of multiple species using the fast Fourier transform. We demonstrate the quantitative determination of chemical concentrations in a ternary mixture. Furthermore, two imaging applications are pursued: (1) quantitative determination of oil content as well as pigment and protein concentration in microalgae cultures; and (2) 3D high-resolution imaging of blood, lipids, and protein distribution in ex vivo mouse skin tissue. We believe that quantitative multiplex SRS uniquely combines the advantage of fast label-free imaging with the fingerprinting capability of Raman spectroscopy and enables numerous applications in lipid biology as well as biomedical imaging.
Published Version: doi:10.1021/ja210081h
Other Sources: http://bernstein.harvard.edu/papers/ja210081h.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:10875725
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