Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy
Author
Pernik, Douglas R.
Holtom, Gary
Published Version
https://doi.org/10.1021/ja210081hMetadata
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Fu, Dan, Fa-Ke Lu, Xu Zhang, Christian Freudiger, Douglas R. Pernik, Gary Holtom, and Xiaoliang Sunney Xie. 2012. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. Journal of the American Chemical Society 134(8): 3623-3626.Abstract
Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations have been limited to single Raman band measurements. We present a novel modulation multiplexing approach that allows real-time detection of multiple species using the fast Fourier transform. We demonstrate the quantitative determination of chemical concentrations in a ternary mixture. Furthermore, two imaging applications are pursued: (1) quantitative determination of oil content as well as pigment and protein concentration in microalgae cultures; and (2) 3D high-resolution imaging of blood, lipids, and protein distribution in ex vivo mouse skin tissue. We believe that quantitative multiplex SRS uniquely combines the advantage of fast label-free imaging with the fingerprinting capability of Raman spectroscopy and enables numerous applications in lipid biology as well as biomedical imaging.Other Sources
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