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dc.contributor.authorFu, Dan
dc.contributor.authorLu, Fake
dc.contributor.authorZhang, Xu
dc.contributor.authorFreudiger, Christian Wilhelm
dc.contributor.authorPernik, Douglas R.
dc.contributor.authorHoltom, Gary
dc.contributor.authorXie, Xiaoliang Sunney
dc.date.accessioned2013-07-16T19:51:29Z
dc.date.issued2012
dc.identifier.citationFu, Dan, Fa-Ke Lu, Xu Zhang, Christian Freudiger, Douglas R. Pernik, Gary Holtom, and Xiaoliang Sunney Xie. 2012. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. Journal of the American Chemical Society 134(8): 3623-3626.en_US
dc.identifier.issn0002-7863en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:10875725
dc.description.abstractStimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations have been limited to single Raman band measurements. We present a novel modulation multiplexing approach that allows real-time detection of multiple species using the fast Fourier transform. We demonstrate the quantitative determination of chemical concentrations in a ternary mixture. Furthermore, two imaging applications are pursued: (1) quantitative determination of oil content as well as pigment and protein concentration in microalgae cultures; and (2) 3D high-resolution imaging of blood, lipids, and protein distribution in ex vivo mouse skin tissue. We believe that quantitative multiplex SRS uniquely combines the advantage of fast label-free imaging with the fingerprinting capability of Raman spectroscopy and enables numerous applications in lipid biology as well as biomedical imaging.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.description.sponsorshipEngineering and Applied Sciencesen_US
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofdoi:10.1021/ja210081hen_US
dc.relation.hasversionhttp://bernstein.harvard.edu/papers/ja210081h.pdfen_US
dash.licenseOAP
dc.subjectin-vivoen_US
dc.subjectCARS microscopyen_US
dc.subjectSRS microscopyen_US
dc.subjectsensitivityen_US
dc.titleQuantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopyen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalJournal of the American Chemical Societyen_US
dash.depositing.authorXie, Xiaoliang Sunney
dc.date.available2013-07-16T19:51:29Z
dc.identifier.doi10.1021/ja210081h*
dash.contributor.affiliatedZhang, Xu
dash.contributor.affiliatedLu, Fake
dash.contributor.affiliatedFreudiger, Christian Wilhelm
dash.contributor.affiliatedFu, Dan
dash.contributor.affiliatedXie, Xiaoliang


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